Rapid diagnosis of tuberculosis directly from clinical specimens using a gene chip

被引：13

作者：

Chang, H. J. ^{[1
]}

Huang, M. Y. ^{[2
,3
,4
]}

Yeh, C. S. ^{[1
,5
,6
]}

Chen, C. C. ^{[1
,5
,6
]}

Yang, M. J. ^{[2
]}

Sun, C. S. ^{[2
]}

Lee, C. K. ^{[7
]}

Lin, S. R. ^{[1
,5
,6
]}

机构：

[1] Fooyin Univ, Biomed Technol Dev Ctr, Ta Liao Hsiang 831, Kaohsiung Hsien, Taiwan

[2] Kaohsiung Med Univ, Coll Med, Fac Med, Grad Inst Med, Kaohsiung, Taiwan

[3] Kaohsiung Med Univ, Coll Med, Fac Med, Dept Radiat Oncol, Kaohsiung, Taiwan

[4] Kaohsiung Med Univ Hosp, Dept Radiat Oncol, Kaohsiung, Taiwan

[5] Fooyin Univ, Sch Med & Hlth Sci, Dept Med Technol, Ta Liao Hsiang 831, Kaohsiung Hsien, Taiwan

[6] Dept Med Res, Fooyin, Taiwan

[7] Fooyin Univ Hosp, Dept Internal Med, Div Infect Dis, Pingtung, Taiwan

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2010年 / 16卷 / 08期

关键词：

Gene chip; membrane array methods; rapid diagnosis; sputum; tuberculosis; MYCOBACTERIUM-TUBERCULOSIS; COLORECTAL-CANCER; EARLY RELAPSE; COMPLEX; BACILLUS; IDENTIFICATION; POLYMORPHISM; PREVALENCE; EVOLUTION;

D O I：

10.1111/j.1469-0691.2009.03045.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

P>The aim of this study was to explore a gene chip capable of detecting the presence of Mycobacterium tuberculosis isolates directly in clinical sputum specimens and to compare it with current molecular detection techniques. At first, we selected 13 M. tuberculosis-specific target genes to construct a gene chip for rapid diagnosis. Using the membrane array method, we diagnosed M. tuberculosis by gene chip directly from 246 sputum specimens from patients suspected of having tuberculosis. Among 80 M. tuberculosis complex (MTBC) culture-positive sputum specimens, the MTBC detection rate was 62.5% (50/80) by PCR-restriction fragment length polymorphism (RFLP), 70% (56/80) by acid-fast staining, and 85% (68/80) by the membrane array method. Furthermore, subspecies showed different gene expression patterns in the membrane array. In conclusion, MTBC could be detected directly in sputum by the membrane array method. The rapidity of detection and the capability of differentiating subspecies could make this method useful in the control and prevention of tuberculosis.

引用

页码：1090 / 1096

页数：7

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