One-Step Preparation of Reduction-Responsive Biodegradable Polymers as Efficient Intracellular Drug Delivery Platforms

被引：19

作者：

Cai, Tongjiang ^{[1
]}

Chen, Yangjun ^{[1
]}

Wang, Yin ^{[1
]}

Wang, Haibo ^{[1
]}

Liu, Xiangsheng ^{[1
]}

Jin, Qiao ^{[1
]}

Agarwal, Seema ^{[2
]}

Ji, Jian ^{[1
]}

机构：

[1] Zhejiang Univ, MOE Key Lab Macromol Synth & Functionalizat, Minist Educ, Dept Polymer Sci & Engn, Hangzhou 310027, Peoples R China

[2] Univ Bayreuth, Bayreuth Ctr Colloids & Interfaces, D-95440 Bayreuth, Germany

来源：

MACROMOLECULAR CHEMISTRY AND PHYSICS | 2014年 / 215卷 / 19期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

biodegradable; drug release; 2-methylene-1; 3-dioxepane (MDO); reduction-responsive; RING-OPENING POLYMERIZATION; BLOCK-COPOLYMERS; NANOPARTICLES; MICELLES; PH; ASSEMBLIES; CHEMISTRY; 2-METHYLENE-1,3-DIOXEPANE; FUNCTIONALIZATION; POLYESTERS;

D O I：

10.1002/macp.201400311

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Reduction-responsive biodegradable polymeric micelles based on functional 2-methylene-1,3-dioxepane (MDO) copolymers are developed and investigated for triggered doxorubicin (DOX) release. The MDO-based copolymers P(MDO-co-PEGMA-co-PDSMA) are synthesized via the simple one-step radical ring-opening copolymerization of MDO, poly(ethylene glycol) methyl ether methacrylate (PEGMA), and pyridyldisulfide ethylmethacrylate (PDSMA). The copolymers can self-assemble to form micelles in aqueous solution. DOX, a model anticancer drug, is loaded into the micelles with the drug loading content (DLC) of 11.3%. The micelles can be disassembled under a reductive environment (10 x 10(-3)m glutathione), which results in a triggered drug release behavior. The glutathione-mediated intracellular drug release of DOX-loaded micelles is investigated against A549 cells. Confocal laser scanning microscopy (CLSM) results demonstrated that DOX-loaded micelles exhibits faster drug release in glutathione monoester (GSH-OEt)-pretreated A549 cells, compared with untreated and buthionine sulfoximine (BSO)-pretreated A549 cells. Based on the facile synthetic strategy, the reduction-sensitive biodegradable micelles with triggered intracellular drug release are promising for anticancer drug delivery.

引用

页码：1848 / 1854

页数：7

共 39 条

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