Multiplexed Protein Patterns on a Photosensitive Hydrophilic Polymer Matrix

被引：18

作者：

Bhatnagar, Parijat ^{[1
]}

Malliaras, George G. ^{[3
]}

Kim, Il ^{[4
]}

Batt, Carl A. ^{[2
]}

机构：

[1] Cornell Univ, Dept Biomed Engn, Ithaca, NY 14853 USA

[2] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA

[3] Cornell Univ, Dept Mat Sci & Engn, Ithaca, NY 14853 USA

[4] Pusan Natl Univ, Dept Polymer Sci & Engn, Pusan 609735, South Korea

来源：

ADVANCED MATERIALS | 2010年 / 22卷 / 11期

基金：

美国国家科学基金会;

关键词：

ON-A-CHIP; LABEL-FREE; IMMUNOLOGICAL SYNAPSE; DRUG DISCOVERY; TECHNOLOGIES; LITHOGRAPHY; MICROFLUIDICS; ADSORPTION; THROUGHPUT; BIOSENSORS;

D O I：

10.1002/adma.200903255

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A photosensitive hydrogel film containing a photocleavable protecting group is attached to silicon oxide. Projection photolithography is used to generate carboxylic groups in selective patterns on this film, to which proteins are coupled through their primary amine groups. The carboxylic groups on the proteins are chemically protected and the process is repeated to sequentially pattern additional proteins on the hydrogel surface.

引用

页码：1242 / +

页数：6

共 60 条

[1] PHOTOSENSITIVE PROTECTING GROUPS OF AMINO-SUGARS AND THEIR USE IN GLYCOSIDE SYNTHESIS - 2-NITROBENZYLOXYCARBONYLAMINO AND 6-NITROVERATRYLOXYCARBONYLAMINO DERIVATIVES [J].

AMIT, B ;

ZEHAVI, U ;

PATCHORN.A .

JOURNAL OF ORGANIC CHEMISTRY, 1974, 39 (02) :192-196

[2] Stereolithography of three-dimensional bioactive poly(ethylene glycol) constructs with encapsulated cells [J].

Arcaute, Karina ;

Mann, Brenda K. ;

Wicker, Ryan B. .

ANNALS OF BIOMEDICAL ENGINEERING, 2006, 34 (09) :1429-1441

[3] Dendrimer-scaffold-based electron-beam patterning of biomolecules [J].

Bhatnagar, P ;

Mark, SS ;

Kim, I ;

Chen, HY ;

Schmidt, B ;

Lipson, M ;

Batt, CA .

ADVANCED MATERIALS, 2006, 18 (03) :315-+

[4] Integrated reactive ion etching to pattern cross-linked hydrophilic polymer structures for protein immobilization [J].

Bhatnagar, Parijat ;

Strickland, Aaron D. ;

Kim, Il ;

Malliaras, George G. ;

Batt, Carl A. .

APPLIED PHYSICS LETTERS, 2007, 90 (14)

[5]

Bochet CG, 2001, ANGEW CHEM INT EDIT, V40, P2071, DOI 10.1002/1521-3773(20010601)40:11<2071::AID-ANIE2071>3.0.CO

[6]

2-9

[7] PHOTOGENERATION OF ORGANIC-BASES FROM ORTHO-NITROBENZYL-DERIVED CARBAMATES [J].

CAMERON, JF ;

FRECHET, JMJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (11) :4303-4313

[8] Optical biosensors in drug discovery [J].

Cooper, MA .

NATURE REVIEWS DRUG DISCOVERY, 2002, 1 (07) :515-528

[9] Non-optical screening platforms: the next wave in label-free screening? [J].

Cooper, Matthew A. .

DRUG DISCOVERY TODAY, 2006, 11 (23-24) :1068-1074

[10] Future lab-on-a-chip technologies for interrogating individual molecules [J].

Craighead, Harold .

NATURE, 2006, 442 (7101) :387-393

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