Discovery and characterization of miRNA during cellular senescence in bone marrow-derived human mesenchymal stem cells

被引:41
作者
Yoo, Jung Ki [1 ]
Kim, Chang-Hyun [2 ]
Jung, Ho Yong [1 ]
Lee, Dong Ryul [3 ]
Kim, Jin Kyeoung [1 ]
机构
[1] CHA Univ, Coll Pharm, Dept Pharm, Songnam 463836, Gyeonggi Do, South Korea
[2] Dongguk Ilsan Hosp, Coll Med, Gyeonggi Do 410773, South Korea
[3] CHA Univ, Coll Med, CHA Gangnam Med Ctr, Fertil Ctr, Seoul 135081, South Korea
基金
新加坡国家研究基金会;
关键词
MSC; miRNA; LAMC1; Cellular senescence; EXTRACELLULAR-MATRIX; EXPRESSION PROFILES; GROWTH ARREST; STROMAL CELLS; LIFE-SPAN; CANCER; MICRORNAS; PATHWAY; FAMILY; GENES;
D O I
10.1016/j.exger.2014.07.020
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
Cellular senescence is an irreversible cell cycle arrest in which specific mRNAs and miRNAs are involved in senescence progression. miRNAs interact with specific mRNAs to regulate various cellular mechanisms, including metabolism, proliferation, apoptosis, senescence and differentiation. In this study, we identify and characterize miRNAs during cellular senescence in mesenchymal stem cells (MSCs). Using previously reported miRNAs, expression profiling of 23 miRNAs was performed using real-time PCR analysis. Among these miRNAs, 19 miRNAs showed upregulated expression patterns in senescent MSCs compared with young MSCs, and 5 miRNAs were downregulated. These miRNAs have not been previously identified as being related to cellular senescence but seem to be related. miR-103-2*, miR-140-5p and miR-330-5p are highly upregulated, while miR-29b and miR-199b-5p are significantly downregulated in senescent MSCs. We identify unique functions of 5 miRNAs and predict putative target genes of 5 miRNAs using our previous report. Among them, miR-199b-5p directly suppressed LAMC1 expression, as shown in a luciferase assay. miR-199b-5p significantly regulates translational activity but does not control post-transcriptional activity. Likewise, miR-199b-5p modulates LAMC networks, which demonstrates the resulting phenomenon during cellular senescence, namely, that miR-199b-5p indirectly regulates cellular senescence in MSCs. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:139 / 145
页数:7
相关论文
共 48 条
  • [1] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004)
    Bartel, David P.
    [J]. CELL, 2007, 131 (04) : 11 - 29
  • [2] Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion
    Baxter, MA
    Wynn, RF
    Jowitt, SN
    Wraith, JE
    Fairbairn, LJ
    Bellantuono, I
    [J]. STEM CELLS, 2004, 22 (05) : 675 - 682
  • [3] The genetics of cellular senescence
    Bérubé, NG
    Smith, JR
    Pereira-Smith, OM
    [J]. AMERICAN JOURNAL OF HUMAN GENETICS, 1998, 62 (05) : 1015 - 1019
  • [4] HOW DOES THE EXTRACELLULAR-MATRIX DIRECT GENE-EXPRESSION
    BISSELL, MJ
    HALL, HG
    PARRY, G
    [J]. JOURNAL OF THEORETICAL BIOLOGY, 1982, 99 (01) : 31 - 68
  • [5] MiRNA Profile Associated with Replicative Senescence, Extended Cell Culture, and Ectopic Telomerase Expression in Human Foreskin Fibroblasts
    Bonifacio, Laura N.
    Jarstfer, Michael B.
    [J]. PLOS ONE, 2010, 5 (09): : 1 - 8
  • [6] miR-199a-5p Is Upregulated during Fibrogenic Response to Tissue Injury and Mediates TGFbeta-Induced Lung Fibroblast Activation by Targeting Caveolin-1
    Cardenas, Christian Lacks Lino
    Henaoui, Imene Sarah
    Courcot, Elisabeth
    Roderburg, Christoph
    Cauffiez, Christelle
    Aubert, Sebastien
    Copin, Marie-Christine
    Wallaert, Benoit
    Glowacki, Francois
    Dewaeles, Edmone
    Milosevic, Jadranka
    Maurizio, Julien
    Tedrow, John
    Marcet, Brice
    Lo-Guidice, Jean-Marc
    Kaminski, Naftali
    Barbry, Pascal
    Luedde, Tom
    Perrais, Michael
    Mari, Bernard
    Pottier, Nicolas
    [J]. PLOS GENETICS, 2013, 9 (02):
  • [7] Colognato H, 2000, DEV DYNAM, V218, P213, DOI 10.1002/(SICI)1097-0177(200006)218:2<213::AID-DVDY1>3.0.CO
  • [8] 2-R
  • [9] High levels of oncomiR-21 contribute to the senescence-induced growth arrest in normal human cells and its knock-down increases the replicative lifespan
    Dellago, Hanna
    Preschitz-Kammerhofer, Barbara
    Terlecki-Zaniewicz, Lucia
    Schreiner, Carina
    Fortschegger, Klaus
    Chang, Martina W. -F.
    Hackl, Matthias
    Monteforte, Rossella
    Kuehnel, Harald
    Schosserer, Markus
    Gruber, Florian
    Tschachler, Erwin
    Scheideler, Marcel
    Grillari-Voglauer, Regina
    Grillari, Johannes
    Wieser, Matthias
    [J]. AGING CELL, 2013, 12 (03) : 446 - 458
  • [10] A BIOMARKER THAT IDENTIFIES SENESCENT HUMAN-CELLS IN CULTURE AND IN AGING SKIN IN-VIVO
    DIMRI, GP
    LEE, XH
    BASILE, G
    ACOSTA, M
    SCOTT, C
    ROSKELLEY, C
    MEDRANO, EE
    LINSKENS, M
    RUBELJ, I
    PEREIRASMITH, O
    PEACOCKE, M
    CAMPISI, J
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) : 9363 - 9367