Chitosan stabilized gold nanoparticle based electrochemical ractopamine immunoassay

被引：34

作者：

He, Linghao ^{[1
]}

Guo, Chuanpan ^{[1
]}

Song, Yingpan ^{[1
]}

Zhang, Shuai ^{[1
]}

Wang, Minghua ^{[1
]}

Peng, Donglai ^{[1
]}

Fang, Shaoming ^{[1
]}

Zhang, Zhihong ^{[1
]}

Liu, Chun-Sen ^{[1
]}

机构：

[1] Zhengzhou Univ Light Ind, Henan Prov Key Lab Surface & Interface Sci, 136 Sci Ave, Zhengzhou 450001, Henan, Peoples R China

来源：

MICROCHIMICA ACTA | 2017年 / 184卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Cyclic voltammetry; Electrochemical impedance spectroscopy; Immunosensor; Plasma-assisted method; Nanoparticles; Nanogold; Real sample; REDUCED GRAPHENE OXIDE; QUANTUM DOTS; IMPEDIMETRIC APTASENSOR; COLORIMETRIC DETECTION; AU NANOPARTICLES; IMMUNOSENSOR; FLUORESCENCE; CLENBUTEROL; SALBUTAMOL; SENSOR;

D O I：

10.1007/s00604-017-2315-z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The authors describe an electrochemical immunoassay for the beta-adrenergic agonist ractopamine (RAC). It is based on the use of chitosan-stabilized gold nanoparticles (chit-AuNPs). Antibodies against RAC (anti-RAC) readily adsorb on the surface of the chit-AuNPs due to their high bio-affinity. The amount of chitosan used to synthesize chit-AuNPs does not affect the size and distribution of the NPs but amplifies the electrochemical signal of the electrode. The assay has a detection limit as low as 2.3 pg.mL(-1) (equivalent to a 6.7 fM concentration), with a response that is linear in the 0.01 to 5 ng.mL(-1) RAC concentration range. The assay is selective, acceptably reproducible, stable, and well applicable to the detection of RAC.

引用

页码：2919 / 2924

页数：6

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