Prevention of seizures and reorganization of hippocampal functions by transplantation of bone marrow cells in the acute phase of experimental epilepsy

被引:53
作者
Costa-Ferro, Zaquer S. M. [1 ,2 ,3 ,4 ]
Vitola, Affonso S. [1 ,2 ]
Pedroso, Michele F. [1 ,2 ]
Cunha, Fernanda B. [1 ,2 ]
Xavier, Leder L. [6 ]
Machado, Denise C. [1 ,5 ]
Soares, Milena B. P. [4 ,7 ]
Ribeiro-dos-Santos, Ricardo [4 ,7 ]
daCosta, Jaderson C. [1 ,2 ,3 ]
机构
[1] Pontificia Univ Catolica Rio Grande do Sul, Inst Cerebro, BR-90610000 Porto Alegre, RS, Brazil
[2] Pontificia Univ Catolica Rio Grande do Sul, Inst Pesquisas Biomed, Lab Neurociencias, BR-90610000 Porto Alegre, RS, Brazil
[3] Univ Fed Rio Grande do Sul, Dept Fisiol, Inst Ciencias Basicas Saude, Porto Alegre, RS, Brazil
[4] Fundacao Oswaldo Cruz, Ctr Pesquisas Goncalo Moniz, Lab Engn Tecidual & Imunofarmacol, Salvador, BA, Brazil
[5] Pontificia Univ Catolica Rio Grande do Sul, Ctr Terapia Celular, Inst Pesquisas Biomed, BR-90610000 Porto Alegre, RS, Brazil
[6] Pontificia Univ Catolica Rio Grande do Sul, Fac Biociencias, Dept Ciencias Fisiol, BR-90610000 Porto Alegre, RS, Brazil
[7] Hosp Sao Rafael, Ctr Biotecnol & Terapia Celular, Salvador, BA, Brazil
来源
SEIZURE-EUROPEAN JOURNAL OF EPILEPSY | 2010年 / 19卷 / 02期
关键词
Bone marrow cells; Epilepsy; Temporal lobe; Experimental status epilepticus; Neuronal loss; TEMPORAL-LOBE EPILEPSY; SPONTANEOUS RECURRENT SEIZURES; LONG-LASTING POTENTIATION; LITHIUM-PILOCARPINE; STEM-CELLS; SYNAPTIC-TRANSMISSION; MICROGLIAL RESPONSE; TERM POTENTIATION; PROGENITOR CELLS; STROMAL CELLS;
D O I
10.1016/j.seizure.2009.12.003
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
In this study, we investigated the therapeutic potential of bone marrow mononuclear cells (BMCs) in a model of epilepsy induced by pilocarpine in rats. BMCs obtained from green fluorescent protein (GFP) transgenic mice or rats were transplanted intravenously after induction Of Status epilepticus (SE). Spontaneous recurrent seizures (SRS) were monitored using Racine's seizure severity scale. All of the rats in the saline-treated epileptic control group developed SRS, whereas none of the BMC-treated epileptic animals had seizures in the short term (15 days after transplantation), regardless of the BMC Source. Over the long-term chronic phase ( 120 days after transplantation), only 25% of BMC-treated epileptic animals had seizures, but with a lower frequency and do ration compared to the epileptic control group. The density of hippocampal neurons in the brains of animals treated with BMCs was markedly preserved. At hippocampal Schaeffer collateral-CA1 synapses, long-term potentiation was preserved in BMC-transplanted rats compared to epileptic Controls. The donor-derived GFP(+) cells were rarely found in the brains of transplanted epileptic rats. In conclusion, treatment with BMCs can prevent the development of chronic seizures, reduce neuronal loss, and influence the reorganization of the hippocampal neuronal network. (C) 2010 Published by Elsevier Ltd on behalf of British Epilepsy Association.
引用
收藏
页码:84 / 92
页数:9
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