Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli:: Cell aggregation and biofilm formation

被引:141
作者
Sherlock, O
Schembri, MA
Reisner, A
Klemm, P
机构
[1] Tech Univ Denmark, Bioctr, Ctr Biomed Microbiol, DK-2800 Lyngby, Denmark
[2] Univ Queensland, Sch Mol & Microbial Sci, Dept Microbiol & Parasitol, Brisbane, Qld, Australia
关键词
D O I
10.1128/JB.186.23.8058-8065.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Diarrhea-causing Escherichia coli strains are responsible for numerous cases of gastrointestinal disease and constitute a serious health problem throughout the world. The ability to recognize and attach to host intestinal surfaces is an essential step in the pathogenesis of such strains. AIDA is a potent bacterial adhesin associated with some diarrheagenic E. coli strains. AIDA mediates bacterial attachment to a broad variety of human and other mammalian cells. It is a surface-displayed autotransporter protein and belongs to the selected group of bacterial glycoproteins; only the glycosylated form binds to mammalian cells. Here, we show that AIDA possesses self-association characteristics and can mediate autoaggregation of E. coli cells. We demonstrate that intercellular AIDA-AIDA interaction is responsible for bacterial autoaggregation. Interestingly, AIDA-expressing cells can interact with antigen 43 (Ag43) -expressing cells, which is indicative of an intercellular AIDA-Ag43 interaction. Additionally, AIDA expression dramatically enhances biofilm formation by E. coli on abiotic surfaces in How chambers.
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页码:8058 / 8065
页数:8
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