Formation of the Dynamic Clusters in Concentrated Lysozyme Protein Solutions

被引:142
|
作者
Porcar, Lionel [2 ]
Falus, Peter [2 ]
Chen, Wei-Ren [3 ]
Faraone, Antonio [1 ,4 ]
Fratini, Emiliano [5 ]
Hong, Kunlun [6 ]
Baglioni, Piero [5 ]
Liu, Yun [1 ,7 ]
机构
[1] Natl Inst Stand & Technol, NCNR, Gaithersburg, MD 20899 USA
[2] ILL, F-38042 Grenoble 9, France
[3] Oak Ridge Natl Lab, Spallat Neutron Source, NSS Div, Oak Ridge, TN 37831 USA
[4] Univ Maryland, Dept Mat Sci & Engn, College Pk, MD 20742 USA
[5] Univ Florence, Dept Chem & CSGI, I-50019 Florence, Italy
[6] Oak Ridge Natl Lab, CNMS, Oak Ridge, TN 37831 USA
[7] Univ Delaware, Dept Chem Engn, Newark, DE 19716 USA
来源
JOURNAL OF PHYSICAL CHEMISTRY LETTERS | 2010年 / 1卷 / 01期
关键词
SELF-DIFFUSION COEFFICIENTS; HIGH-FREQUENCY VISCOSITY; EQUILIBRIUM CLUSTERS; NEUTRON-SCATTERING; DISPERSIONS; SUSPENSIONS; PARTICLES; COLLOIDS; PHASE;
D O I
10.1021/jz900127c
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Neutron spin echo (NSE) and small angle neutron scattering (SANS) were used to investigate the correlation between structure and short-time dynamics of lysozyme solutions in the presence of protein clusters as previously reported. It was found that, upon increasing protein concentration, the. self-diffusion coefficient at the short time limit becomes much smaller than that of the corresponding hard-sphere and charged colloidal suspensions at the same volume fraction. Contrary to literature conclusions, we find that, at relatively low concentrations, the system consists mostly of monomers or dimers; while, at high concentrations, large dynamic clusters dominate. Our results will benefit the understanding of colloidal systems with both a short-range attraction and an electrostatic repulsion that are ubiquitous in many biologically relevant systems.
引用
收藏
页码:126 / 129
页数:4
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