Hippocampal γ-aminobutyric acid transporter alterations following focal epileptogenesis induced in rat amygdala

被引:21
作者
Ueda, Y
Willmore, LJ
机构
[1] Miyazaki Med Coll, Dept Psychiat, Kiyotake, Miyazaki 8891692, Japan
[2] Univ Texas, Sch Med, Dept Neurol, Houston, TX USA
关键词
GABA; GABA transporters; epilepsy; western blot; FeCl3;
D O I
10.1016/S0361-9230(00)00272-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
To study the role of hippocampal gamma-aminobutyric acid (GABA) transporters in epileptogenesis, we induced chronic seizures by ferric cation injection into the rat amygdaloid body, We used western blotting to measure alterations in the expression of hippocampal GABA transporter proteins GAT-1 and GAT-3, GAT-1 increased bilaterally (from 150 to 250%) at 5 to 15 days after injection, but returned to control levels by 30 days. In contrast, GAT-3 also significantly increased bilaterally at 5 days and 15 days, but remained elevated bilaterally at 30 days after injection, Alterations in GAT-1 levels are apparently transient responses to seizure activity that occur during the acute phase of epileptogenesis, However, we propose that regulation of the GAT-3 subtype transporter was chronically elevated (at least 30 days), represents an effect of epileptogenesis induced by ferric ion injected into the amygdaloid body. (C) 2000 Elsevier Science Inc.
引用
收藏
页码:357 / 361
页数:5
相关论文
共 36 条
[1]   Reduction of GABA and glutamate transporter messenger RNAs in the severe-seizure genetically epilepsy-prone rat [J].
Akbar, MT ;
Rattray, M ;
Williams, RJ ;
Chong, NWS ;
Meldrum, BS .
NEUROSCIENCE, 1998, 85 (04) :1235-1251
[2]   NEUROTRANSMITTER TRANSPORTERS - RECENT PROGRESS [J].
AMARA, SG ;
KUHAR, MJ .
ANNUAL REVIEW OF NEUROSCIENCE, 1993, 16 :73-93
[3]  
ATWELL D, 1993, NEURON, V11, P401
[4]  
BABB TL, 1989, J NEUROSCI, V9, P2562
[5]  
BORDEN LA, 1995, RECEPTOR CHANNEL, V3, P129
[6]  
BORDEN LA, 1992, J BIOL CHEM, V267, P21098
[7]   FUNCTIONAL EXPRESSION AND CNS DISTRIBUTION OF A BETA-ALANINE-SENSITIVE NEURONAL GABA TRANSPORTER [J].
CLARK, JA ;
DEUTCH, AY ;
GALLIPOLI, PZ ;
AMARA, SG .
NEURON, 1992, 9 (02) :337-348
[8]   AN [NA+ +K+]COUPLED L-GLUTAMATE TRANSPORTER PURIFIED FROM RAT-BRAIN IS LOCATED IN GLIAL-CELL PROCESSES [J].
DANBOLT, NC ;
STORMMATHISEN, J ;
KANNER, BI .
NEUROSCIENCE, 1992, 51 (02) :295-310
[9]   HIPPOCAMPAL GABA TRANSPORTER FUNCTION IN TEMPORAL-LOBE EPILEPSY [J].
DURING, MJ ;
RYDER, KM ;
SPENCER, DD .
NATURE, 1995, 376 (6536) :174-177
[10]   LOCALIZATION OF MESSENGER-RNAS ENCODING 3 GABA TRANSPORTERS IN RAT-BRAIN - AN IN-SITU HYBRIDIZATION STUDY [J].
DURKIN, MM ;
SMITH, KE ;
BORDEN, LA ;
WEINSHANK, RL ;
BRANCHEK, TA ;
GUSTAFSON, EL .
MOLECULAR BRAIN RESEARCH, 1995, 33 (01) :7-21