The involvement of alpha-melanocyte-stimulating hormone in the hyperpigmentation of human skin autografts

被引:0
作者
Xue, Chun-Yu [1 ]
Li, Li [1 ]
Guo, Ling-Li [1 ]
Li, Jun-Hui [1 ]
Xing, Xin [1 ]
机构
[1] Second Mil Med Univ, Changhai Hosp, Dept Plast Surg, Shanghai 200433, Peoples R China
基金
中国国家自然科学基金;
关键词
alpha-Melanocyte-stimulating hormone; Human skin autografts; Hyperpigmentation; Melanocyte; Pro-opiomelanocortin; IN-VITRO; PIGMENTATION; MELANIN; GRAFT; MSH; MELANOGENESIS; EXPRESSION; PEPTIDES; COLOR;
D O I
10.1016/j.burns.2009.04.025
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Hyperpigmentation occurs frequently in human skin autografts, resulting in an unsatisfactory appearance. Objective: This study aims to elucidate the role of alpha-melanocyte-stimulating hormone (alpha-MSH) in the hyperpigmentation process of skin autografts by analysing the expression of alpha-MSH and the amount of melanin in human autografted skin and normal skin. Methods: Immunohistochemistry and reverse transcription-polymerase chain reaction methods were carried out to detect the expression and distribution of alpha-MSH and proopiomelanocortin (POMC) in skin autografts, including full-thickness skin autografts, split-thickness skin autografts and normal skin. The Fontana-Masson stain was used to detect the amount of melanin in all the skin specimens. Results: The level of alpha-MSH and POMC expression in autografted skin was much higher than that of control normal skin, and thinner split-thickness skin autografts expressed higher levels of POMC mRNA and alpha-MSH protein. The amount of melanin in skin autografts was significantly higher compared with normal skin. The expression of alpha-MSH correlated well with the amount of melanin in skin autograft epidermis. Conclusions: These results indicate that the function of melanogenesis, transfer of melanin in melanocytes, was dramatically enhanced in skin autografts by alpha-MSH. The over-expression of alpha-MSH may play an important role in the hyperpigmentation process of skin autografts and provide a novel mechanism of this process. (C) 2009 Elsevier Ltd and ISBI. All rights reserved.
引用
收藏
页码:284 / 290
页数:7
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