IL-1β induced changes in hypothalamic IL-1R1 and IL-1R2 mRNA expression in the rat

被引：12

作者：

Parker, LC ^{[1
]}

Rushforth, DA ^{[1
]}

Rothwell, NJ ^{[1
]}

Luheshi, GN ^{[1
]}

机构：

[1] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England

来源：

MOLECULAR BRAIN RESEARCH | 2000年 / 79卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

rat; IL-1; beta; receptor; hypothalamus; RT-PCR;

D O I：

10.1016/S0169-328X(00)00099-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Interleukin-l receptor (IL-1R1 and IL-1R2) mRNA expression was detected within the rat hypothalamus, a primary site of IL-I action, using RT-PCR. Levels of expression were unchanged by cardiac saline-perfusion. However. intracerebroventricular (i.c.v.) administration of IL-IP caused changes in receptor mRNA expression in non-perfused animals that were profoundly different to those observed in their saline-perfused counterparts. This study demonstrates the importance of perfusing tissue to remove blood cells when determining changes in IL-1 receptor mRNA expression. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：156 / 158

页数：3

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