IL-1β induced changes in hypothalamic IL-1R1 and IL-1R2 mRNA expression in the rat

被引:12
作者
Parker, LC [1 ]
Rushforth, DA [1 ]
Rothwell, NJ [1 ]
Luheshi, GN [1 ]
机构
[1] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England
来源
MOLECULAR BRAIN RESEARCH | 2000年 / 79卷 / 1-2期
基金
英国医学研究理事会;
关键词
rat; IL-1; beta; receptor; hypothalamus; RT-PCR;
D O I
10.1016/S0169-328X(00)00099-1
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Interleukin-l receptor (IL-1R1 and IL-1R2) mRNA expression was detected within the rat hypothalamus, a primary site of IL-I action, using RT-PCR. Levels of expression were unchanged by cardiac saline-perfusion. However. intracerebroventricular (i.c.v.) administration of IL-IP caused changes in receptor mRNA expression in non-perfused animals that were profoundly different to those observed in their saline-perfused counterparts. This study demonstrates the importance of perfusing tissue to remove blood cells when determining changes in IL-1 receptor mRNA expression. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:156 / 158
页数:3
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