Ab initio reconstruction and experimental design for cryo electron microscopy

被引：62
作者：
Doerschuk, PC [1 ]
Johnson, JE
机构：
[1] Purdue Univ, Sch Elect & Comp Engn, W Lafayette, IN 47907 USA
[2] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
来源：
IEEE TRANSACTIONS ON INFORMATION THEORY | 2000年 / 46卷 / 05期
基金：
美国国家科学基金会;
关键词：
Cramer-Rao bounds; cryo electron microscopy; expectation-maximization algorithm; statistical inverse problems; statistical reconstruction problems; viruses;
D O I：
10.1109/18.857786
中图分类号：
TP [自动化技术、计算机技术];
学科分类号：
0812 ;
摘要：
A statistical model for the object and the complete image formation process in cryo electron microscopy of viruses is presented. Using this model, maximum-likelihood reconstructions of the three-dimensional (3-D) structure of viruses are computed using the expectation max-imitation algorithm, and alternative experimental designs are evaluated based on Cramer-Rao bounds. Numerical examples of the reconstructions and experimental design evaluations are provided based on Cowpea mosaic virus.
引用
收藏
页码：1714 / 1729
页数：16
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