Noncompetitive activation of the peroxidase-catalyzed oxidation of o-phenylenediamine by melamine

被引：4

作者：

Karaseva, EI ^{[1
]}

Naumchik, IV ^{[1
]}

Metelitza, DI ^{[1
]}

机构：

[1] Natl Acad Sci Belarus, Inst Bioorgan Chem, Minsk 220141, BELARUS

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2003年 / 29卷 / 01期

关键词：

horseradish peroxidase; activation; inhibition; o-phenylenediamine; melamine; peroxidase activation; enzymatic assay; nucleophilic catalysis;

D O I：

10.1023/A:1022278402568

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peroxidase-catalyzed oxidation of o-phenylenediamine (PDA) is greatly activated with melamine (MA) in 15 mM phosphate-citrate buffer at pH 6.0-7.4 in a noncompetitive manner: k(cat) and K-m increase in direct proportion to the MA concentration. An extent of the activation is quantitatively characterized with a coefficient alpha (in M-1), which essentially increases along with the rise in pH from 6.0 to 7.4. MA acts as a nucleophilic catalyst in the oxidation process: it most likely affects the peroxidase active site from the distal position of heme. MA noncompetitively inhibits the peroxidase oxidation of PDA at pH 4.3, since it completely loses its nucleophilic properties in acidic medium. A rapid, highly accurate, and simple analytical test system based on the kinetics of melamine-activated oxidation of PDA is proposed for the quantitative determination of melamine within the concentration range of 1 of 10(-4) 10(-3) M. This test system uses the spectrophotometric determination of the PDA oxidation product at 455 nm.

引用

页码：43 / 49

页数：7

共 35 条

[21]

METELITSA DI, 1991, IZV AKAD NAUK BSS KN, P75

[22]

METELITSA DI, 1984, MODELIROVANIE OKISLI

[23] PEROXIDASE-CATALYZED COOXIDATION OF HALOGEN-SUBSTITUTED PHENOLS AND 4-AMINOANTIPYRINE [J].

METELITZA, DI ;

LITVINCHUK, AV ;

SAVENKOVA, MI .

JOURNAL OF MOLECULAR CATALYSIS, 1991, 67 (03) :401-411

[24] NUCLEAR MAGNETIC-RESONANCE STUDIES OF HEMOPROTEINS - ACID-ALKALINE TRANSITION, LIGAND-BINDING CHARACTERISTICS, AND STRUCTURE OF HEME ENVIRONMENTS IN HORSERADISH-PEROXIDASE [J].

MORISHIMA, I ;

OGAWA, S ;

INUBUSHI, T ;

YONEZAWA, T ;

IIZUKA, T .

BIOCHEMISTRY, 1977, 16 (23) :5109-5115

[25]

POLAC JM, 1984, INTRO IMMUNOCYTOCHEI

[26]

PUCHKAEV AV, 1993, THESIS MINSK

[27]

PUCHKAEV AV, 1992, IZV AKAD NAUK BSSR K, P78

[28] Chemiluminescent biosensors based on porous supports with immobilized peroxidase [J].

Rubtsova, MY ;

Kovba, GV ;

Egorov, AM .

BIOSENSORS & BIOELECTRONICS, 1998, 13 (01) :75-85

[29]

*SOV ENTS, 1964, KRATK KHIM ENTS, V3, P108

[30] 2,3-DIAMINOPHENAZINE IS THE PRODUCT FROM THE HORSERADISH PEROXIDASE-CATALYZED OXIDATION OF ORTHO-PHENYLENEDIAMINE [J].

TARCHA, PJ ;

CHU, VP ;

WHITTERN, D .

ANALYTICAL BIOCHEMISTRY, 1987, 165 (01) :230-233

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