aac(6′)-Ib-cr Genotyping by Simultaneous High-Resolution Melting Analyses of an Unlabeled Probe and Full-Length Amplicon

被引：13
作者：
Bell, Jan M.
Turnidge, John D. [2 ,3 ,4 ]
Andersson, Patiyan [1 ]
机构：
[1] Womens & Childrens Hosp, Dept Microbiol & Infect Dis, SA Pathol, Adelaide, SA 5006, Australia
[2] Univ Adelaide, Dept Pathol, Adelaide, SA 5000, Australia
[3] Univ Adelaide, Dept Paediat, Adelaide, SA 5000, Australia
[4] Univ Adelaide, Dept Mol & Biomed Sci, Adelaide, SA 5000, Australia
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2010年 / 54卷 / 03期
关键词：
CIPROFLOXACIN-MODIFYING ENZYME; KLEBSIELLA-PNEUMONIAE; CLINICAL ISOLATE; BETA-LACTAMASE; PREVALENCE; DETERMINANTS; KOREA; GENE; QNR;
D O I：
10.1128/AAC.01476-09
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6')-Ib-cr that is capable of distinguishing between the two genetic aac(6 ')-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.
引用
收藏
页码：1378 / 1380
页数：3
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