The clonal composition of Mycobacterium tuberculosis in clinical specimens could be modified by culture

被引:44
作者
Martin, Ana [1 ]
Herranz, Marta [1 ]
Ruiz Serrano, Maria Jesus [1 ]
Bouza, Emilio [1 ]
Garcia de Viedma, Dario [1 ]
机构
[1] Hosp Gen Univ Gregorio Maranon, Serv Microbiol Clin & Enfermedades Infecciosas, Madrid, Spain
关键词
Tuberculosis; Clonal complexity; Genotyping; MIRU-VNTR; EXOGENOUS REINFECTION; MIXED INFECTION; RESISTANCE; STRAINS; HETEROGENEITY; POLYMORPHISM;
D O I
10.1016/j.tube.2010.03.012
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background: The application of molecular tools has revealed that infection by Mycobacterium tuberculosis (MTB) is more complex than initially assumed. Genotyping is generally performed on cultures. However, there is no information about bacterial clonal complexity in clinical specimens or whether standard culture procedures can modify this complexity. Methods: An in vitro assay was performed to determine whether culture can modify the clonal complexity of the MTB population in clinical specimens. Pairs of MTB strains (10 pairs) or stain-positive sputa (4 pairs) were mixed in different volumetric proportions. The DNA extracted from the mixtures before and after culture was genotyped using mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis to detect potential changes in the proportion of the mixed strains. Results: In 6/10 pairs of MTB strains and 2/4 pairs of sputa, marked changes were observed in clonal composition after culture, even in mixtures of strains differing in their drug-susceptibility patterns. In some cases, only one of the mixed strains was detected after culture. Conclusions: The initial clonal composition in bacteriologically complex clinical specimens could be underestimated if genotyping analysis is performed after culture. Genotyping strategies aimed at analyzing clinical samples must be optimized to reveal the real dimension of clonal complexity in infection by MTB. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:201 / 207
页数:7
相关论文
共 28 条
  • [1] TRANSMISSION OF TUBERCULOSIS IN NEW-YORK-CITY - AN ANALYSIS BY DNA-FINGERPRINTING AND CONVENTIONAL EPIDEMIOLOGIC METHODS
    ALLAND, D
    KALKUT, GE
    MOSS, AR
    MCADAM, RA
    HAHN, JA
    BOSWORTH, W
    DRUCKER, E
    BLOOM, BR
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1994, 330 (24) : 1710 - 1716
  • [2] [Anonymous], ARCH INTERN MED
  • [3] Molecular epidemiology study of exogenous reinfection in an area with a low incidence of tuberculosis
    Bandera, A
    Gori, A
    Catozzi, L
    Esposti, AD
    Marchetti, G
    Molteni, C
    Ferrario, G
    Codecasa, L
    Penati, V
    Matteelli, A
    Franzetti, F
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (06) : 2213 - 2218
  • [4] In vivo phenotypic dominance in mouse mixed infections with Mycobacterium tuberculosis clinical isolates
    Barczak, AK
    Domenech, P
    Boshoff, HIM
    Reed, MB
    Manca, C
    Kaplan, G
    Barry, CE
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2005, 192 (04) : 600 - 606
  • [5] Physiological cost of rifampin resistance induced in vitro in Mycobacterium tuberculosis
    Billington, OJ
    McHugh, TD
    Gillespie, SH
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (08) : 1866 - 1869
  • [6] Simultaneous infection with multiple strains of Mycobacterium tuberculosis
    Braden, CR
    Morlock, GP
    Woodley, CL
    Johnson, KR
    Colombel, AC
    Cave, MD
    Yang, ZH
    Valway, SE
    Onorato, IM
    Crawford, JT
    [J]. CLINICAL INFECTIOUS DISEASES, 2001, 33 (06) : E42 - E47
  • [7] BURNENS AP, 1989, ZBL BAKT-INT J MED M, V271, P85
  • [8] Exogenous reinfection with tuberculosis on a European island with a moderate incidence of disease
    Caminero, JA
    Pena, MJ
    Campos-Herrero, MI
    Rodríguez, JC
    Afonso, O
    Martin, C
    Pavón, JM
    Torres, MJ
    Burgos, M
    Cabrera, P
    Small, PM
    Enarson, DA
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 163 (03) : 717 - 720
  • [9] Evidence of exogenous reinfection and mixed infection with more than one strain of Mycobacterium tuberculosis among Spanish HIV-infected inmates
    Chaves, F
    Dronda, F
    Alonso-Sanz, M
    Noriega, AR
    [J]. AIDS, 1999, 13 (05) : 615 - 620
  • [10] Genetic heterogeneity in Mycobacterium tuberculosis isolates reflected IS6110 restriction fragment length polymorphism patterns as low-intensity bands
    de Boer, AS
    Kremer, K
    Borgdorff, MW
    de Haas, PEW
    Heersma, HF
    van Soolingen, D
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (12) : 4478 - 4484