Human tuberculosis brain promotes neuronal apoptosis but not in astrocytes with high expression of vascular endothelial growth factor

被引：3

作者：

Othman, Fatihah N. ^{[1
]}

Muthuraju, Sangu ^{[1
]}

Noor, Siti Suraiya M. ^{[2
]}

Abdullah, Sarimah ^{[3
]}

Yusoff, Abdul Aziz Mohd ^{[1
]}

Tharakan, John ^{[1
]}

Bhaskar, Shalini ^{[1
]}

Mahmood, Mohd Shah ^{[4
]}

Kassim, Fauziah ^{[4
]}

Rafia, Hanip ^{[4
]}

Haspani, Mohd Saffari Mohd ^{[4
]}

Alias, Azmi ^{[4
]}

Pando, Rogelio H. ^{[5
]}

Abdullah, Jafri M. ^{[1
]}

Jaafar, Hasnan ^{[6
]}

机构：

[1] Univ Sains Malaysia, Dept Neurosci, Jalan Hosp Univ Sains Malaysia, Kota Baharu 16150, Kelantan, Malaysia

[2] Univ Sains Malaysia, Dept Microbiol, Kota Baharu, Malaysia

[3] Univ Sains Malaysia, Unit Biostat & Res Methodol, Kubang Kerian, Malaysia

[4] Kuala Lumpur Hosp, Dept Forens & Pathol, Kuala Lumpur, Malaysia

[5] Natl Inst Med Sci & Nutr Salvador Zubiran, Dept Pathol, Mexico City, DF, Mexico

[6] Univ Sains Malaysia, Dept Pathol, Kota Baharu 16150, Kelantan, Malaysia

来源：

TUBERCULOSIS | 2018年 / 112卷

关键词：

Human brain tuberculosis; Astrocyte; Neurons; Apoptosis; VEGF; Bcl2 and Bax; MYCOBACTERIUM-TUBERCULOSIS; INFECTION; MODEL; VEGF; HYDROCEPHALUS; PATHWAY;

D O I：

10.1016/j.tube.2018.07.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The present study aimed to investigate the involvement of the angiogenic marker vascular endothelia growth factor (VEGF) and apoptotic markers of Bcl-2 and Bax in the neurons and astrocytes in the brain infected by Mycobacterium tuberculosis. The immunohistochemistry staining was performed to analyze the expression of the VEGF, Bcl-2 and Bax in the astrocytes and neurons. The expression of VEGF was high in neurons and astrocytes in both the infected brain and control tissues with no difference of angiogenic activity (p = 0.40). Higher Bcl-2 expression was seen in astrocytes of infected brain tissues compared to the control tissues (p = 0.004) promoted a higher anti-apoptotic activity in astrocytes. The neurons expressed strong Bax expression in the infected brain tissues compared to the control tissues (p < 0.001), which indicated more apoptosis in neurons. Thus, neuronal death and survival of infected astrocytes together with high expression of VEGF might be associated with formation of brain tuberculosis. In conclusion, neurons could be more vulnerable than astrocytes in human tuberculosis brain with high expression of VEGF.

引用

页码：45 / 51

页数：7

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