Antiviral property and mode of action of a sulphated polysaccharide from Sargassum patens against herpes simplex virus type 2

A sulphated polysaccharide (SP2) was isolated from the brown alga Sargassum patens. SP2 inhibited the replication of herpes simplex virus type 2 (HSV-2) dose-dependently by 38.5-96.1% of the control level, after incubations with 0.78-12.5 mug/ml of the polysaccharide. SP2 exhibited extracellular virucidal activity only in high concentrations (greater than or equal to12.5 mug/ml) but significantly inhibited the virus attachment to its host cells by 45.1%, at concentration as low as 1 mug/ml. All the results from this study suggested that the antiviral mode of action of SP2 could be ascribed to the inhibition of virus adsorption, which is different from that of the current drug of choice acyclovir. (C) 2004 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.