Silibinin induces the generation of nitric oxide in human breast cancer MCF-7 cells

被引：27

作者：

Wang, Hong-Jun ^{[1
]}

Wei, Xiao-Feng ^{[1
]}

Jiang, Yuan-Yuan ^{[1
]}

Huang, Huai ^{[1
]}

Yang, Ying ^{[1
]}

Fan, Si-Miao ^{[1
]}

Zang, Ling-He ^{[1
]}

Tashiro, Shin-Ichi ^{[2
]}

Onodera, Satoshi ^{[2
]}

Ikejima, Takashi ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, China Japan Res Inst Med & Pharmaceut Sci, Shenyang 110016, Liaoning Prov, Peoples R China

[2] Showa Pharmaceut Univ, Dept Clin & Biomed Sci, Tokyo 1948543, Japan

来源：

FREE RADICAL RESEARCH | 2010年 / 44卷 / 05期

关键词：

RAT-HEART; INHIBITION; PEROXYNITRITE; SYNTHASE; OXYGEN; THERAPEUTICS; MITOCHONDRIA; ACTIVATION;

D O I：

10.3109/10715761003692495

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Increasing research has concentrated on the anti-tumour efficacy of silibinin, a flavonolignan that is clinically used as an hepatoprotectant. However, previous work has found that silibinin-induced apoptosis is accompanied by protective superoxide (O-2 center dot-) generation in MCF-7 cells. This study further reports the formation of reactive nitrogen species (RNS) in the same system. It finds that silibinin induces nitric oxide (center dot NO) generation in a time- and concentration-dependent manner. Moreover, the results support that there exists an inter-regulation pattern between RNS and reactive oxygen species (ROS) generation. In addition, silibinin is also found to induce RNS and ROS generation in the isolated populations of mouse peripheral blood mononuclear cells (PBMCs) and a simple in vivo model of Caenorhabditis elegans.

引用

页码：577 / 584

页数：8

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