Real-time analysis of the cytopathic effect of African swine fever virus

被引：9

作者：

Burmakina, Galina ^{[1
]}

Bliznetsov, Kirill ^{[2
]}

Malogolovkin, Alexander ^{[1
]}

机构：

[1] FRCVM, Bakulova 1, Volginskiy 601125, Russia

[2] LLC Alamed, Moscow, Russia

来源：

JOURNAL OF VIROLOGICAL METHODS | 2018年 / 257卷

基金：

俄罗斯科学基金会; 美国食品与农业研究所;

关键词：

Real-time cell analysis system; African swine fever virus; CELL ANALYSIS;

D O I：

10.1016/j.jviromet.2018.04.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Conventional methods, which quantitatively assess virus replication, are based on direct examination of N cytopathic effect (CPE), which is time consuming, tedious and based on endpoint reading. The Real-Time Ce Analysis (RTCA) xCELLigence((R)) system offers an alternative approach to evaluate virus-induced CPE, and her was evaluated as a means to dynamically assess CPE caused by African swine fever virus (ASFV). RTCA was use to identify optimum time for ASFV infection based on cell index (CI) and to evaluate ASFV CPE kinetics in COS-cells. Data indicated that the RTCA has tremendous potential to methodologically and quantitatively improv assays used to study efficiency of ASFV drug inhibitors and neutralizing antibodies.

引用

收藏

页码：58 / 61

页数：4

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