Antigenotoxicity and antimutagenicity of ethanolic extracts of Brazilian green propolis and its main botanical source determined by the Allium cepa test system

被引：5

作者：

Roberto, Matheus Mantuanelli ^{[1
]}

Jamal, Claudia Masrouah ^{[2
]}

Malaspina, Osmar ^{[3
]}

Marin-Morales, Maria Aparecida ^{[1
]}

机构：

[1] Univ Estadual Paulista, Inst Biociencias, Dept Biol, Rio Claro, SP, Brazil

[2] Univ Fed Espirito Santo, Centro Ciencias Saude, Dept Ciencias Farmaceut, Vitoria, ES, Spain

[3] Univ Estadual Paulista, Ctr Estudo Insetos Sociais, Rio Claro, SP, Brazil

来源：

GENETICS AND MOLECULAR BIOLOGY | 2016年 / 39卷 / 02期

关键词：

chromosomal aberration; micronucleus; anticlastogenicity; protective effects; flavonoids; BIOLOGICAL-PROPERTIES; MERISTEMATIC CELLS; PLANT-ORIGIN; IN-VIVO; MUTAGENICITY; GENOTOXICITY; FLAVONOIDS; CHEMOPREVENTION; INHIBITION; MECHANISMS;

D O I：

10.1590/1678-4685-GMB-2015-0130

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Brazilian green propolis is a resinous substance prepared by bees from parts of the plant Baccharis dracunculifolia. As it possess several biological properties, this work assessed the cytotoxic/anticytotoxic, genotoxic/antigenotoxic and mutagenic/antimutagenic potential of ethanolic extracts of Brazilian green propolis (EEGP) and of B. dracunculifolia (EEBD), by means of the Allium cepa test system. The effects were evaluated by assessing the chromosomal aberrations (CA) and micronuclei (MN) frequencies on meristematic and F1 generation cells from onion roots. Chemical analyses performed with the extracts showed differences in flavonoid quality and quantity. No genotoxic or mutagenic potential was detected, and both extracts were capable of inhibiting cellular damage caused by methyl methanesulfonate (MMS) treatment, reducing the frequencies of CA and MN. By these data, we can infer that, independent of their flavonoid content, the extracts presented a protective effect in A. cepa cells against the clastogenicity of MMS.

引用

页码：257 / 269

页数：13

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