Involvement of UL24 in herpes-simplex-virus-1-induced dispersal of nucleolin

被引:68
作者
Lymberopoulos, Maria H. [1 ]
Pearson, Angela [1 ]
机构
[1] Univ Quebec, Inst Armand Frappier, INRS Energie, Laval, PQ H7V 1B7, Canada
关键词
UL24; nucleolus; nucleolin; fibrillarin; herpes simplex virus 1;
D O I
10.1016/j.virol.2007.01.028
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
UL24 of herpes simplex virus 1 is important for efficient viral replication, but its function is unknown. We generated a recombinant virus, vHA-UL24, encoding UL24 with an N-terminal hemagglutinin tag. By indirect immunofluorescence at 9 h post-infection (hpi), we detected HAUL24 in nuclear foci and in cytoplasmic speckles. HA-UL24 partially co-localized with nucleolin, but not with ICP8 or coilin, markers for nucleoli, viral replication compartments, and Cajal bodies respectively. HA-UL24 staining was often juxtaposed to that of another nucleolar protein, fibrillarin. Analysis of HSV-1-induced nucleolar modifications revealed that by 18 hpi, nucleolin staining had dispersed, and fibrillarin staining went from clusters of small spots to a few separate but prominent spots. Fibrillarin redistribution appeared to be independent of UL24. In contrast, cells infected with a UL24-deficient virus retained foci of nucleolin staining. Our results demonstrate involvernent of UL24 in dispersal of nucleolin during infection. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:397 / 409
页数:13
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