Involvement of UL24 in herpes-simplex-virus-1-induced dispersal of nucleolin

被引：68

作者：

Lymberopoulos, Maria H. ^{[1
]}

Pearson, Angela ^{[1
]}

机构：

[1] Univ Quebec, Inst Armand Frappier, INRS Energie, Laval, PQ H7V 1B7, Canada

来源：

VIROLOGY | 2007年 / 363卷 / 02期

关键词：

UL24; nucleolus; nucleolin; fibrillarin; herpes simplex virus 1;

D O I：

10.1016/j.virol.2007.01.028

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

UL24 of herpes simplex virus 1 is important for efficient viral replication, but its function is unknown. We generated a recombinant virus, vHA-UL24, encoding UL24 with an N-terminal hemagglutinin tag. By indirect immunofluorescence at 9 h post-infection (hpi), we detected HAUL24 in nuclear foci and in cytoplasmic speckles. HA-UL24 partially co-localized with nucleolin, but not with ICP8 or coilin, markers for nucleoli, viral replication compartments, and Cajal bodies respectively. HA-UL24 staining was often juxtaposed to that of another nucleolar protein, fibrillarin. Analysis of HSV-1-induced nucleolar modifications revealed that by 18 hpi, nucleolin staining had dispersed, and fibrillarin staining went from clusters of small spots to a few separate but prominent spots. Fibrillarin redistribution appeared to be independent of UL24. In contrast, cells infected with a UL24-deficient virus retained foci of nucleolin staining. Our results demonstrate involvernent of UL24 in dispersal of nucleolin during infection. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：397 / 409

页数：13

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