Development of 10 single-copy nuclear DNA markers for Euchresta horsfieldii (Fabaceae), a rare medicinal plant

被引：2

作者：

Priyadi, Arief ^{[1
,2
,3
]}

Feng, Chao ^{[2
]}

Kang, Ming ^{[2
]}

Huang, Hongwen ^{[2
]}

机构：

[1] Indonesian Inst Sci LIPI, Bali Bot Garden, Tabanan 82191, Bali, Indonesia

[2] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

APPLICATIONS IN PLANT SCIENCES | 2018年 / 6卷 / 09期

关键词：

Euchresta horsfieldii; Fabaceae; genetic diversity; RNA-Seq; single-copy nuclear DNA (scnDNA) markers; POPULATION-GENETICS; SOFTWARE;

D O I：

10.1002/aps3.1178

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

PREMISE OF THE STUDY: Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single-copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure. METHODS AND RESULTS: We performed RNA-Seq and de novo assembly of the transcriptome. Ten primer sets were developed for E. horsfieldii, all of which also amplified in E. japonica and E. tubulosa. CONCLUSIONS: These scnDNA markers will be an important resource for the study of genetic diversity and population structure of E. horsfieldii and other species in the genus Euchresta.

引用

页数：4

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