Early response of neural stem/progenitor cells after X-ray irradiation in vitro

被引：18
作者：
Kato, Tomoaki
Kanemura, Yonehiro
Shiraishi, Kazunori
Miyake, Jun
Kodama, Seiji
Hara, Masayuki
机构：
[1] Osaka Prefecture Univ, Grad Sch Sci, Dept Biol Sci, Naka Ku, Sakai, Osaka 5998570, Japan
[2] Osaka Prefecture Univ, Grad Sch Agr, Dept Appl Biochem, Sakai, Osaka 5998570, Japan
[3] Natl Hosp Org, Osaka Natl Hosp, Inst Clin Res, Chuo Ku, Osaka, Japan
[4] Natl Inst Adv Ind Sci & Technol, Res Inst Cell Engn, Amagasaki, Hyogo, Japan
来源：
NEUROREPORT | 2007年 / 18卷 / 09期
关键词：
cdc2; cell cycle; laser scanning cytometer; neural stem cell; Notch; p53; X-ray; STEM-CELLS; SPATIOTEMPORAL ACTIVATION; IONIZING IRRADIATION; PRECURSOR CELLS; DNA-DAMAGE; NEUROGENESIS; BRAIN; PHOSPHORYLATION; PROLIFERATION; INVOLVEMENT;
D O I：
10.1097/WNR.0b013e3281053c34
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We investigated the effect of oxidative stress on cell cycle regulation of neural stem/progenitor cells in neurosphere culture. We exposed murine neural stem/progenitor cells to 2 Gy of X-ray irradiation at 48 h after first passage. We found that G2 and GI-arrested cells increased at 3 and 12h after X-ray irradiation, respectively by using laser scanning cytometer. We revealed that such G2 and G1 arrests were correlated with phosphorylation of cdc2 and p53, respectively by Western blotting analysis. Furthermore, we found that the effects of X-ray irradiation of neural stem/progenitor cells involved inactivation of Notch signal. These results suggest that the drastic response of neural stem/ progenitor cells after X-ray irradiation occurred even in the short period.
引用
收藏
页码：895 / 900
页数：6
相关论文
共 23 条
  • [1] Cell cycle kinetics of expanding populations of neural stem and progenitor cells in vitro
    Alam, S
    Sen, A
    Behie, LA
    Kallos, MS
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 2004, 88 (03) : 332 - 347
  • [2] A unified hypothesis on the lineage of neural stem cells
    Alvarez-Buylla, A
    García-Verdugo, JM
    Tramontin, AD
    [J]. NATURE REVIEWS NEUROSCIENCE, 2001, 2 (04) : 287 - 293
  • [3] Cognitive sequelae of brain tumor treatment
    Byrne, TN
    [J]. CURRENT OPINION IN NEUROLOGY, 2005, 18 (06) : 662 - 666
  • [4] Phosphorylation of murine p53 at Ser-18 regulates the p53 responses to DNA damage
    Chao, C
    Saito, S
    Anderson, CW
    Appella, E
    Xu, Y
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) : 11936 - 11941
  • [5] Chojnacki A, 2003, J NEUROSCI, V23, P1730
  • [6] DNA-DAMAGE TRIGGERS A PROLONGED P53-DEPENDENT G(1) ARREST AND LONG-TERM INDUCTION OF CIP1 IN NORMAL HUMAN FIBROBLASTS
    DI LEONARDO, A
    LINKE, SP
    CLARKIN, K
    WAHL, GM
    [J]. GENES & DEVELOPMENT, 1994, 8 (21) : 2540 - 2551
  • [7] Notch controls proliferation and differentiation of stem cells in a dose-dependent manner
    Guentchev, M
    McKay, RDG
    [J]. EUROPEAN JOURNAL OF NEUROSCIENCE, 2006, 23 (09) : 2289 - 2296
  • [8] Visualization of spatiotemporal activation of Notch signaling: Live monitoring and significance in neural development
    Kohyama, J
    Tokunaga, A
    Fujita, Y
    Miyoshi, H
    Nagai, T
    Miyawaki, A
    Nakao, K
    Matsuzaki, Y
    Okano, H
    [J]. DEVELOPMENTAL BIOLOGY, 2005, 286 (01) : 311 - 325
  • [9] Radiation response of neural precursor cells: Linking cellular sensitivity to cell cycle checkpoints, apoptosis and oxidative stress
    Limoli, CL
    Giedzinski, E
    Rola, R
    Otsuka, S
    Palmer, TD
    Fike, JR
    [J]. RADIATION RESEARCH, 2004, 161 (01) : 17 - 27
  • [10] Increased activity and involvement of caspase-3 in radiation-induced apoptosis in neural cells precursors from developing rat brain
    Michelin, S
    Perez, MD
    Dubner, D
    Gisone, P
    [J]. NEUROTOXICOLOGY, 2004, 25 (03) : 387 - 398
123