LncRNA FOXD2-AS1 regulates chondrocyte proliferation in osteoarthritis by acting as a sponge of miR-206 to modulate CCND1 expression

被引：55
作者：
Cao, Lei [1 ]
Wang, Yang [2 ,3 ]
Wang, Qiugen [1 ]
Huang, Jianhua [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Dept Trauma & Orthoped, 650 New Songjiang Rd, Shanghai 201620, Peoples R China
[2] Second Mil Med Univ, Changhai Hosp, Dept Emergency, Shanghai 200433, Peoples R China
[3] Second Mil Med Univ, Changhai Hosp, Dept Orthopaed, Shanghai 200433, Peoples R China
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2018年 / 106卷
关键词：
Osteoarthritis; Long non-coding RNAs; FOXD2-AS1; miR-206; CCND1; COMPETING ENDOGENOUS RNA; CYCLIN D1; SIGNALING PATHWAY; LONG; CANCER; PROGRESSION; APOPTOSIS; PROMOTES; KNEE; KNOCKDOWN;
D O I：
10.1016/j.biopha.2018.07.048
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Recently, accumulating evidence demonstrated that the long non-coding RNAs (lncRNAs) play important roles in osteoarthritis (OA) progression. However, the role of lncRNA FOXD2-AS1 on OA is still unclear. In the present study, qRT-PCR showed that expression of FOXD2-AS1 and Cyclin Dl (CCND1) was upregulated in OA cartilage tissues, while miR-206 expression was significantly decreased. CCK-8 and colony formation assays showed that FOXD2-AS1 could promote chondrocytes viability. Flow cytometry analysis showed that FOXD2-AS1 inhibition arrested chondrocytes in G0/G1 phase and induced cells apoptosis. Furthermore, luciferase reporter assay and RIP assay showed that FOXD2-AS1 could function as a sponge of miR-206. Rescue assays showed that miR-206 inhibitors reversed the effects of FOXD2-AS1 suppression on chondrocytes viability. In addition, we identified that CCND1 acted as a direct target of miR-206. FOXD2-AS1 suppression could inhibit CCND1 expression in chondrocytes, while miR-206 inhibitors reversed CCND1 expression. Moreover, rescue assays indicated that CCND1 overexpression reversed the effects of FOXD2-AS1 suppression on chondrocytes viability. Taken together, these data indicated that FOXD2-AS1 could promote the growth of chondrocytes by targeting miR-206/CCND1 axis.
引用
收藏
页码：1220 / 1226
页数：7
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