Down-regulation of lipoprotein lipase increases glucose uptake in L6 muscle cells

被引：6

作者：

Lopez, Veronica ^{[1
]}

Saraff, Kumuda ^{[1
]}

Medh, Jheem D. ^{[1
]}

机构：

[1] Calif State Univ Northridge, Dept Chem & Biochem, Northridge, CA 91330 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 389卷 / 01期

基金：

美国国家卫生研究院;

关键词：

PPAR-gamma; Ciglitazone; Lipoprotein lipase; Skeletal muscle; Rat L6 cells; Glucose uptake; siRNA; ACTIVATED RECEPTOR-GAMMA; INSULIN-RESISTANCE; PPAR-GAMMA; OVEREXPRESSION; TROGLITAZONE; METABOLISM; GENE;

D O I：

10.1016/j.bbrc.2009.08.081

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thiazolidinediones (TZDs) are synthetic hypoglycemic agents used to treat type 2 diabetes. TZDs target the peroxisome proliferator activated receptor-gamma (PPAR-gamma) and improve systemic insulin sensitivity. The contributions of specific tissues to TZD action, or the downstream effects of PPAR-gamma activation, are not very clear. We have used a rat skeletal muscle cell line (L6 cells) to demonstrate that TZDs directly target PPAR-gamma in muscle cells. TZD treatment resulted in a significant repression of lipoprotein lipase (LPL) expression in L6 cells. This repression correlated with an increase in glucose uptake. Down-regulation of LPL message and protein levels using siRNA resulted in a similar increase in insulin-dependent glucose uptake. Thus, LPL down-regulation improved insulin sensitivity independent of TZDs. This finding provides a novel method for the management of insulin resistance. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：34 / 39

页数：6

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