Functional Thyroid Follicular cells Differentiation from human-induced Pluripotent stem cells in suspension culture

被引：15

作者：

Arauchi, Ayumi ^{[1
]}

Matsuura, Katsuhisa ^{[1
,2
]}

Shimizu, Tatsuya ^{[1
]}

Okano, Teruo ^{[1
]}

机构：

[1] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Tokyo, Japan

[2] Tokyo Womens Med Univ, Dept Cardiol, Tokyo, Japan

来源：

FRONTIERS IN ENDOCRINOLOGY | 2017年 / 8卷

关键词：

induced pluripotent stem cells; differentiation; 3D-cell culture; thyroid transcription factor; thyroid follicular cells; EFFICIENT DIFFERENTIATION; DEFINITIVE ENDODERM; HUMAN FIBROBLASTS; PAX8; EXPRESSION; GENERATION; GLAND; LUNG; TRANSPLANTATION; ORGANOGENESIS;

D O I：

10.3389/fendo.2017.00103

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The replacement of regenerated thyroid follicular cells (TFCs) is a promising therapeutic strategy for patients with hypothyroidism. Here, we have succeeded in inducing functional TFCs from human-induced pluripotent stem cells (iPSCs) in scalable suspension culture. Differentiation of iPSCs with Activin A treatment produced Sox17- and FoxA2-expressing definitive endodermal cells that also expressed thyroid transcription factors Pax8 and Nkx2-1. Further treatment with thyroid-stimulating hormone (TSH) induced TFCs expressing various types of thyroid proteins including TSH receptor, sodium-iodide symporter, thyroglobulin, and thyroid peroxidase. Interestingly, differentiated cells secreted free thyroxine in vitro. These results indicate successful differentiation of human iPSCs to functional TFCs that may enable us to fabricate thyroid tissues for regenerative medicine and disease models.

引用

页数：11

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