qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

被引：3375

作者：

Hellemans, Jan ^{[1
]}

Mortier, Geert ^{[1
]}

De Paepe, Anne ^{[1
]}

Speleman, Frank ^{[1
]}

Vandesompele, Jo ^{[1
]}

机构：

[1] Ghent Univ Hosp, Ctr Genet Med, B-9000 Ghent, Belgium

来源：

GENOME BIOLOGY | 2007年 / 8卷 / 02期

关键词：

D O I：

10.1186/gb-2007-8-2-r19

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter- run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.

引用

页数：14

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