Detection of proteins on poly(vinylidene difluoride) membranes by scanning electrochemical microscopy

被引：21

作者：

Carano, M ^{[1
]}

Lion, N ^{[1
]}

Abid, JP ^{[1
]}

Girault, HH ^{[1
]}

机构：

[1] EPFL, ISIC, LEPA, CH-1015 Lausanne, Switzerland

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2004年 / 6卷 / 12期

关键词：

protein detection; scanning electrochemical microscopy; SECM; silver nanoparticles; polyvinylidene difluoride; PVDF membrane;

D O I：

10.1016/j.elecom.2004.09.018

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Scanning electrochemical microscopy (SECM) is used in combination with silver nanoparticles tagging to achieve visualization of proteins on surfaces. Proteins are adsorbed on a polyvinylidene difluoride (PVDF) membrane, tagged with silver nanoparticles and then detected by SECM with osmium tris-bipyridine as a redox mediator in aqueous solution. During the SECM scan, the potential is held at a value at which the oxidation of the redox mediator occurs and a positive feedback current is detected when scanning over silver nanoparticles. A negative feedback is observed elsewhere. This method enables protein adsorption mapping on polymeric membranes. (C) 2004 Published by Elsevier B.V.

引用

页码：1217 / 1221

页数：5

共 11 条

[1]

ABID JP, 2003, THESIS SWISS FEDERAL

[2] Colorimetric silver detection of DNA microarrays [J].