In vivo expression of genes in the entomopathogenic fungus Beauveria bassiana during infection of lepidopteran larvae

被引:6
作者
Galidevara, Sandhya [1 ]
Reineke, Annette [2 ]
Koduru, Uma Devi [1 ]
机构
[1] Andhra Univ, Dept Bot, Visakhapatnam 530003, Andhra Pradesh, India
[2] Hsch Geisenheim Univ, Dept Phytomed, Von Lade Str 1, D-65366 Geisenheim, Germany
关键词
Beauveria bassiana; Entomopathogenic fungus; In vivo expression; Pathogenicity genes; qPCR; POLYMERASE CHAIN-REACTION; ANISOPLIAE; RNA; DIVERSIFICATION; QUANTIFICATION; VIRULENCE;
D O I
10.1016/j.jip.2016.03.002
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
The entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin is commercially available as a bio insecticide. The expression of three genes previously identified to have a role in pathogenicity in in vitro studies was validated in vivo in three lepidopteran insects infected with B. bassiana. Expression of all three genes was observed in all the tested insects starting from 48 or 72 h to 10 d post infection corroborating their role in pathogenicity. We suggest that it is essential to test the expression of putative pathogenicity genes both in vitro and in vivo to understand their role in different insect species. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码:32 / 34
页数:3
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