In vivo expression of genes in the entomopathogenic fungus Beauveria bassiana during infection of lepidopteran larvae

被引：6

作者：

Galidevara, Sandhya ^{[1
]}

Reineke, Annette ^{[2
]}

Koduru, Uma Devi ^{[1
]}

机构：

[1] Andhra Univ, Dept Bot, Visakhapatnam 530003, Andhra Pradesh, India

[2] Hsch Geisenheim Univ, Dept Phytomed, Von Lade Str 1, D-65366 Geisenheim, Germany

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2016年 / 136卷

关键词：

Beauveria bassiana; Entomopathogenic fungus; In vivo expression; Pathogenicity genes; qPCR; POLYMERASE CHAIN-REACTION; ANISOPLIAE; RNA; DIVERSIFICATION; QUANTIFICATION; VIRULENCE;

D O I：

10.1016/j.jip.2016.03.002

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

The entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin is commercially available as a bio insecticide. The expression of three genes previously identified to have a role in pathogenicity in in vitro studies was validated in vivo in three lepidopteran insects infected with B. bassiana. Expression of all three genes was observed in all the tested insects starting from 48 or 72 h to 10 d post infection corroborating their role in pathogenicity. We suggest that it is essential to test the expression of putative pathogenicity genes both in vitro and in vivo to understand their role in different insect species. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：32 / 34

页数：3

共 22 条

[1] Reconstructing the diversification of subtilisins in the pathogenic fungus Metarhizium anisopliae [J].

Bagga, S ;

Hu, G ;

Screen, SE ;

St Leger, RJ .

GENE, 2004, 324 :159-169

[2] Secretome of the Biocontrol Agent Metarhizium anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection [J].

Beys-da-Silva, Walter O. ;

Santi, Lucelia ;

Berger, Markus ;

Calzolari, Diego ;

Passos, Dario O. ;

Guimaraes, Jorge A. ;

Moresco, James J. ;

Yates, John R. .

JOURNAL OF PROTEOME RESEARCH, 2014, 13 (05) :2282-2296

[3] Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays [J].

Bustin, SA .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2000, 25 (02) :169-193

[4] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2009, 55 (04) :611-622

[5] Differential expression of genes involved in entomopathogenicity of the fungi Metarhizium anisopliae var. anisopliae and M. anisopliae var. acridum (Clavicipitaceae) [J].

Carneiro-Leao, M. P. ;

Andreote, F. D. ;

Araujo, W. L. ;

Oliveira, N. T. .

GENETICS AND MOLECULAR RESEARCH, 2011, 10 (02) :769-778

[6] Differential gene expression in silkworm in response to Beauveria bassiana infection [J].

Chengxiang, H. ;

Guangxing, Q. ;

Ting, L. ;

Xinglin, M. ;

Rui, Z. ;

Pan, Z. ;

Zhongyuan, S. ;

Xijie, G. .

GENE, 2011, 484 (1-2) :35-42

[7] EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana.: I.: Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia [J].

Cho, Eun-Min ;

Liu, Li ;

Farmerie, William ;

Keyhani, Nemat O. .

MICROBIOLOGY-SGM, 2006, 152 :2843-2854

[8] Ochratoxin A Management in Vineyards by Lobesia botrana Biocontrol [J].

Cozzi, Giuseppe ;

Somma, Stefania ;

Haidukowski, Miriam ;

Logrieco, Antonio F. .

TOXINS, 2013, 5 (01) :49-59

[9]

Devi KU, 2012, BIOTECHNOLOGY OF FUNGAL GENES, P343

[10] A study of host specificity in the entomopathogenic fungus Beauveria bassiana (Hypocreales, Clavicipitaceae) [J].

Devi, K. Uma ;

Padmavathi, J. ;

Rao, C. Uma Maheswara ;

Khan, Akbar Ali P. ;

Mohan, Murali C. .

BIOCONTROL SCIENCE AND TECHNOLOGY, 2008, 18 (10) :975-989

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