Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2

被引：28
|
作者：
McConnell, G
Riis, E
机构：
[1] Univ Strathclyde, Ctr Biophoton, SIBS, Glasgow G4 0NR, Lanark, Scotland
[2] Univ Strathclyde, Dept Phys, Glasgow G4 0NG, Lanark, Scotland
来源：
PHYSICS IN MEDICINE AND BIOLOGY | 2004年 / 49卷 / 20期
关键词：
D O I：
10.1088/0031-9155/49/20/007
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.
引用
收藏
页码：4757 / 4763
页数：7
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