JAK2 and PTPRC mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus

被引：16

作者：

Qian, Danfeng ^{[1
,2
,3
]}

Liu, Lu ^{[1
,2
]}

Zhu, Tingting ^{[1
,2
,3
]}

Wen, Leilei ^{[1
,2
]}

Zhu, Zhengwei ^{[1
,2
]}

Yin, Xianyong ^{[4
]}

Qiu, Ying ^{[5
]}

Wu, Mingshun ^{[1
,2
]}

Li, Xueying ^{[1
,2
]}

Ma, Jie ^{[1
,2
]}

Zhang, Qun ^{[1
,2
]}

Jin, Ling ^{[1
,2
]}

Wang, Xiaomeng ^{[1
,2
]}

Xu, Qiaohu ^{[1
,2
]}

Yang, Sen ^{[1
,2
]}

Sheng, Yujun ^{[1
,2
]}

Zhang, Shengquan ^{[3
]}

Zhang, Xuejun ^{[1
,2
]}

机构：

[1] Anhui Med Univ, Affiliated Hosp 1, Inst Dermatol, 81 Meishan Rd, Hefei 230032, Anhui, Peoples R China

[2] Anhui Med Univ, Affiliated Hosp 1, Dept Dermatol, 81 Meishan Rd, Hefei 230032, Anhui, Peoples R China

[3] Anhui Med Univ, Dept Biochem & Mol Biol, 81 Meishan Rd, Hefei, Anhui, Peoples R China

[4] Univ Michigan, Dept Biostat, Ctr Stat Genet, Ann Arbor, MI 48109 USA

[5] Jining 1 Peoples Hosp, Dept Dermatol, Jining, Shandong, Peoples R China

来源：

CLINICAL RHEUMATOLOGY | 2020年 / 39卷 / 02期

基金：

中国国家自然科学基金;

关键词：

JAK/STAT pathway; JAK2; PTPRC; Quantitative reverse transcription polymerase chain reaction; Systemic lupus erythematosus; T-CELLS; INTERFERON; EPIDEMIOLOGY;

D O I：

10.1007/s10067-019-04778-w

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In this study, we aimed to explore the expression levels of JAK2 and PTPRC in peripheral blood mononuclear cells (PBMCs) from SLE patients and controls, detect the effects of SLE activity on genes mRNA expression, and find the association between genes mRNA expression and clinical manifestations of patients. We performed quantitative real-time PCR (qRT-PCR) to test differences in the expression levels of JAK2 and PTPRC in PBMCs extracted from 135 patients with SLE and 130 healthy controls. Furthermore, we detected the regulatory effect of SNPs on gene expression by expression quantitative trait loci (eQTL). We also tested whether the genes mRNA expression was affected with the SLE activity and analyzed the relationship between genes mRNA expression and clinical manifestations of patients. The mRNA expression levels of JAK2 in SLE patients were significantly higher than those in healthy controls (P = 0.005), and PTPRC mRNA expression levels were significantly decreased (P < 0.001). However, no other statistical significance was detected. We found that the elevated JAK2 mRNA expression and the decreased PTPRC mRNA expression may play suggestive roles in the pathogenesis of SLE.

引用

页码：443 / 448

页数：6

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