Reliable, high-quality suppression of NMR signals arising from water and macromolecules: application to bio-fluid analysis

被引：15

作者：

Aguilar, Juan A. ^{[1
]}

Cassani, Julia ^{[1
,2
]}

Probert, Fay ^{[3
]}

Palace, Jacqueline ^{[4
]}

Claridge, Tim D. W. ^{[5
]}

Botana, Adolfo ^{[6
]}

Kenwright, Alan M. ^{[1
]}

机构：

[1] Univ Durham, Dept Chem, South Rd, Durham DH1 3LE, England

[2] Univ Autnoma Metropolitan, Dept Sistemas Biol, Unidad Xochimilco, Col Villa Quietud 04960, DF, Mexico

[3] Univ Oxford, Dept Pharmacol, Mansfield Rd, Oxford OX1 3QT, England

[4] Univ Oxford, Nuffield Dept Clin Neurosci, John Radcliffe Hosp, Level 3,West Wing,Head Ley Way, Oxford OX3 9DU, England

[5] Univ Oxford, Dept Chem, Chem Res Lab, Mansfield Rd, Oxford OX1 3TA, England

[6] JEOL House, Welwyn Garden City AL7 1LT, Herts, England

来源：

ANALYST | 2019年 / 144卷 / 24期

关键词：

HUMAN SERUM-ALBUMIN; SPIN-ECHO; MAGNETIZATION-TRANSFER; DIFFUSION-COEFFICIENTS; LIGAND-BINDING; LACTATE;

D O I：

10.1039/c9an01005j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Analysis of metabolites in biofluids using nuclear magnetic resonance often requires the suppression of obscuring signals arising from water and macromolecules. This paper analyses the limitations of the pulse sequence most commonly used to achieve such suppression (presat-CPMG) and proposes new pulse sequences that do not share those limitations. The utility of these improved pulse sequences is demonstrated in a metabolomic study of multiple sclerosis (MS) patients.

引用

页码：7270 / 7277

页数：8

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