Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum

被引：80

作者：

Palanisamy, Subramanian ^{[1
]}

Vinosha, Manoharan ^{[1
]}

Manikandakrishnan, Muthushanmugam ^{[1
]}

Anjali, Ravichandran ^{[1
]}

Rajasekar, Periyannan ^{[1
]}

Marudhupandi, Thangapandi ^{[2
]}

Manikandan, Ramar ^{[3
]}

Vaseeharan, Baskaralingam ^{[2
]}

Prabhu, Narayanasamy Marimuthu ^{[1
]}

机构：

[1] Alagappa Univ, Dept Anim Hlth & Management, Dis Control & Prevent Lab, Karaikkudi 630004, Tamil Nadu, India

[2] Alagappa Univ, Dept Anim Hlth & Management, Biomat & Biotechnol Anim Hlth Lab, Karaikkudi 630004, Tamil Nadu, India

[3] Univ Madras, Dept Zool, Guindy Campus, Madras 600025, Tamil Nadu, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 116卷

关键词：

Fucoidan; Antioxidant; Anticancer; IN-VITRO ANTIOXIDANT; SULFATED POLYSACCHARIDES; HUMAN COLON; BIOLOGICAL-ACTIVITY; CHLOROGENIC ACID; TOXIC HEPATITIS; DOWN-REGULATION; BROWN SEAWEEDS; APOPTOSIS; CELLS;

D O I：

10.1016/j.ijbiomac.2018.04.163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study was aimed to evaluate the antioxidant and anticancer potential of fucoidan isolated from Sargassum polycystum. The isolated fucoidan was successfully purified by DEAE cellulose-ion exchange chromatography and dialysis. Totally four active fractions (F1-F4) were collected and explored its chemical constitution by calorimetric assays. Among them, fraction 2 (F2) showed the higher yield percentage, fucose and sulphate content. Further, monosaccharide composition, structural and functional properties of the F2 was analyzed by HPLC, FTIR and NMR. F2 shows highest DPPH radical scavenging activity (55.94 +/- 0.69%), reducing power (0.33 absorbance rate), hydrogen peroxide scavenging activity (71.76 +/- 2.14%) and nitric oxide radical scavenging activity (51.81 +/- 1.04%) at 1000 mu g/ml. The cell viability of MCF-7 and HCT-15 cell lines was proportionate to the concentration of F2 with an estimated IC50 was 20 and 50 mu g/ml respectively. The fluorescence and confocal laser scanning microscopic analysis demonstrated the apoptotic morphological changes and cell mediated death in F2 treated cancer cells. Higher amount of LDH release was found in the F2 treated cancer cells than the control group. Thus, the present finding proved that the isolated F2 encompasses significant antioxidant and anticancer property. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：151 / 161

页数：11

共 76 条

[1] Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways [J].

Aisa, Y ;

Miyakawa, Y ;

Nakazato, T ;

Shibata, H ;

Saito, K ;

Ikeda, Y ;

Kizaki, M .

AMERICAN JOURNAL OF HEMATOLOGY, 2005, 78 (01) :7-14

[2] Fucose-Containing Sulfated Polysaccharides from Brown Seaweeds Inhibit Proliferation of Melanoma Cells and Induce Apoptosis by Activation of Caspase-3 in Vitro [J].

Ale, Marcel Tutor ;

Maruyama, Hiroko ;

Tamauchi, Hidekazu ;

Mikkelsen, Jorn D. ;

Meyer, Anne S. .

MARINE DRUGS, 2011, 9 (12) :2605-2621

[3] Antitumor and antimetastatic activity of fucoidan, a sulfated polysaccharide isolated from the Okhotsk sea Fucus evanescens brown alga [J].

Alekseyenko, T. V. ;

Zhanayeva, S. Ya. ;

Venediktova, A. A. ;

Zvyagintseva, T. N. ;

Kuznetsova, T. A. ;

Besednova, N. N. ;

Korolenko, T. A. .

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2007, 143 (06) :730-732

[4]

[Anonymous], PRACTICAL CLIN ENZYM

[5]

[Anonymous], 1995, J AGR FOOD CHEM

[6]

[Anonymous], INT J PHARM PHARM SC

[7]

[Anonymous], EPIDEMIOL HLTH

[8]

[Anonymous], J FOOD SCI TECHNOL

[9]

[Anonymous], DIG ENDOSC

[10]

[Anonymous], J NEUROL NEUROSCI

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