Differentiation diversity of mouse parthenogenetic embryonic stem cells in chimeric mice

被引：4

作者：

Onodera, Yuta ^{[1
,2
]}

Teramura, Takeshi ^{[1
,3
]}

Ozawa, Madoka ^{[2
]}

Takehara, Toshiyuki ^{[2
]}

Mitani, Tasuku ^{[2
,4
]}

Anzai, Masayuki ^{[2
,4
]}

Sagawa, Norimasa ^{[3
]}

Hamanishi, Chiaki ^{[5
]}

Hosoi, Yoshihiko ^{[2
]}

Fukuda, Kanji ^{[1
,6
]}

机构：

[1] Kinki Univ, Sch Med, Inst Adv Clin Med, Osaka 5898511, Japan

[2] Kinki Univ, Grad Sch Biol Oriented Sci & Technol, Dept Genet Engn, Wakayama 6496493, Japan

[3] Mie Univ, Grad Sch Med, Dept Obstet & Gynecol, Tsu, Mie 5148507, Japan

[4] Kinki Univ, Inst Adv Technol, Wakayama 6420017, Japan

[5] Kinki Univ, Sch Med, Dept Orthopaed Surg, Osaka 5898511, Japan

[6] Kinki Univ, Sch Med, Dept Rehabil Med, Osaka 5898511, Japan

来源：

THERIOGENOLOGY | 2010年 / 74卷 / 01期

关键词：

Parthenogenesis; ES cells; Differentiation; Mitochondrial DNA; Mouse; IMPRINTED GENES; IN-VITRO; GERM-LINE; EXPRESSION; GENOME;

D O I：

10.1016/j.theriogenology.2010.01.024

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recent studies have illustrated multiple differentiation potentials of embryonic stem cells (ESCs), derived from parthenogenetic embryos, to various kinds of cells (all three embryonic germ layers). However, differentiation diversity of the parthenogenetic ESCs (PgESCs) in vivo remains to be elucidated. In the present study, we established mouse PgESC-lines and observed their contribution diversity in vivo by producing chimeric mice using embryos possessing single nucleotide polymorphisms of mitochondrial DNA (mtDNA) as hosts. Based on southern blot analysis using specific probes to detect the SNPs on mtDNA, PgESC-derived mtDNA were contained in many organs such as brain, lung, and heart of the chimeric mouse. We concluded that PgESCs contributed to various internal organs in vivo, and that they were also stably maintained in adult animals. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：135 / 145

页数：11

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