Integration of Flow Cytometry and Single Cell Sequencing

被引：9

作者：

Andreyev, Dmitry S. ^{[1
,2
]}

Zybailov, Boris L. ^{[2
]}

机构：

[1] Univ Tyumen, X BIO Inst, Tyumen 625003, Russia

[2] Univ Arkansas Med Sci, Dept Biochem & Mol Biol, Little Rock, AR 72205 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2020年 / 38卷 / 02期

关键词：

PLATFORM;

D O I：

10.1016/j.tibtech.2019.09.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Integrating cytometric analysis of cells, mitochondria, and other polynucleotide-containing biological particles with high-throughput single particle sequencing would provide an ultimate bioanalytical tool, simultaneously assessing phenotype, functionality, genome, and transcriptome of each particle in a large population. Here, we describe how such integration could be performed by adapting existing, well-established technologies.

引用

页码：133 / 136

页数：5

共 14 条

[1] Simultaneous laser-induced fluorescence and scattering detection of individual particles separated by capillary electrophoresis [J].

Andreyev, Dmitry ;

Arriaga, Edgar A. .

ANALYTICAL CHEMISTRY, 2007, 79 (14) :5474-5478

[2] Fabrication of perforated sub-micron silica shells [J].

Andreyev, Dmitry S. ;

Arriaga, Edgar A. .

SCRIPTA MATERIALIA, 2007, 57 (10) :957-959

[3]

Battersby B.J., DEVICE METHODS DIREC

[4] A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages [J].

Kimmerling, Robert J. ;

Szeto, Gregory Lee ;

Li, Jennifer W. ;

Genshaft, Alex S. ;

Kazer, Samuel W. ;

Payer, Kristofor R. ;

Borrajo, Jacob de Riba ;

Blainey, Paul C. ;

Irvine, Darrell J. ;

Shalek, Alex K. ;

Manalis, Scott R. .

NATURE COMMUNICATIONS, 2016, 7

[5] Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding [J].

Lan, Freeman ;

Demaree, Benjamin ;

Ahmed, Noorsher ;

Abate, Adam R. .

NATURE BIOTECHNOLOGY, 2017, 35 (07) :640-+

[6] Mitochondrial quality control pathways: PINK1 acts as a gatekeeper [J].

Leites, Elvira P. ;

Morais, Vanessa A. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 500 (01) :45-50

[7]

McGrath K.E., 2015, SCIENCE, V349, P999

[8] Cytometric bead array: a multiplexed assay platform with applications in various areas of biology [J].

Morgan, E ;

Varro, R ;

Sepulveda, H ;

Ember, JA ;

Apgar, J ;

Wilson, J ;

Lowe, L ;

Chen, R ;

Shivraj, L ;

Agadir, A ;

Campos, R ;

Ernst, D ;

Gaur, A .

CLINICAL IMMUNOLOGY, 2004, 110 (03) :252-266

[9] Gene expression analysis using oligonucleotide arrays produced by maskless photolithography [J].

Nuwaysir, EF ;

Huang, W ;

Albert, TJ ;

Singh, J ;

Nuwaysir, K ;

Pitas, A ;

Richmond, T ;

Gorski, T ;

Berg, JP ;

Ballin, J ;

McCormick, M ;

Norton, J ;

Pollock, T ;

Sumwalt, T ;

Butcher, L ;

Porter, D ;

Molla, M ;

Hall, C ;

Blattner, F ;

Sussman, MR ;

Wallace, RL ;

Cerrina, F ;

Green, RD .

GENOME RESEARCH, 2002, 12 (11) :1749-1755

[10] Multiplexed quantification of proteins and transcripts in single cells [J].

Peterson, Vanessa M. ;

Zhang, Kelvin Xi ;

Kumar, Namit ;

Wong, Jerelyn ;

Li, Lixia ;

Wilson, Douglas C. ;

Moore, Renee ;

McClanahan, Terrill K. ;

Sadekova, Svetlana ;

Klappenbach, Joel A. .

NATURE BIOTECHNOLOGY, 2017, 35 (10) :936-+

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