Dipeptidyl Peptidase-4 and Adolescent Idiopathic Scoliosis: Expression in Osteoblasts

被引:7
|
作者
Normand, Emilie [1 ,2 ]
Franco, Anita [3 ]
Moreau, Alain [3 ,4 ,5 ]
Marcil, Valerie [1 ,2 ]
机构
[1] St Justine Univ Hosp, Res Ctr, Montreal, PQ H3T 1C5, Canada
[2] Univ Montreal, Dept Nutr, Fac Med, Montreal, PQ H3T 1J4, Canada
[3] St Justine Univ Hosp, Res Ctr, Viscogliosi Lab Mol Genet Musculoskeletal Dis, Montreal, PQ H3T 1C5, Canada
[4] Univ Montreal, Dept Biochem & Mol Med, Fac Med, Montreal, PQ H3T 1J4, Canada
[5] Univ Montreal, Dept Stomatol, Fac Dent, Montreal, PQ H3T 1J4, Canada
来源
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词
GLUCAGON-LIKE PEPTIDE-1; LOW BONE MASS; GUT MICROBIOTA; GENE-EXPRESSION; INSULIN; IV; CELLS; GLP-1; INCRETINS; RECEPTOR;
D O I
10.1038/s41598-017-03310-x
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
It has been proposed that girls with adolescent idiopathic scoliosis (AIS) tend to have a taller stature and a lower body mass index. Energy homeostasis, that is known to affect bone growth, could contribute to these characteristics. In circulation, dipeptidyl peptidase-4 (DPP-4) inactivates glucagon-like peptide-1 (GLP-1), an incretin that promotes insulin secretion and sensitivity. Our objectives were to investigate DPP-4 status in plasma and in osteoblasts of AIS subjects and controls and to evaluate the regulatory role of metabolic effectors on DPP-4 expression. DPP-4 activity was assessed in plasma of 113 girls and 62 age-matched controls. Osteoblasts were isolated from bone specimens of AIS patients and controls. Human cells were incubated with glucose, insulin, GLP-1 and butyrate. Gene and protein expressions were evaluated by RT-qPCR and Western blot. Our results showed 14% inferior plasma DPP-4 activity in AIS patients when compared to healthy controls (P = 0.0357). Similarly, osteoblasts derived from AIS subjects had lower DPP-4 gene and protein expression than controls by 90.5% and 57.1% respectively (P < 0.009). DPP-4 expression was regulated in a different manner in osteoblasts isolated from AIS participants compared to controls. Our results suggest a role for incretins in AIS development and severity.
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页数:11
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