Semaphorin 6A Attenuates the Migration Capability of Lung Cancer Cells via the NRF2/HMOX1 Axis

被引:27
作者
Chen, Li-Han [1 ]
Liao, Che-Yu [2 ]
Lai, Liang-Chuan [3 ,4 ]
Tsai, Mong-Hsun [2 ,4 ,5 ,6 ,7 ]
Chuang, Eric Y. [1 ,6 ,8 ,9 ]
机构
[1] Natl Taiwan Univ, Grad Inst Biomed Elect & Bioinformat, Taipei, Taiwan
[2] Natl Taiwan Univ, Inst Biotechnol, Taipei, Taiwan
[3] Natl Taiwan Univ, Inst Physiol, Taipei, Taiwan
[4] Natl Taiwan Univ, Genome & Syst Biol Degree Program, Taipei, Taiwan
[5] Natl Taiwan Univ, Ctr Biotechnol, Taipei, Taiwan
[6] Natl Taiwan Univ, Ctr Genom Med, Bioinformat & Biostat Core, Taipei, Taiwan
[7] Acad Sinica, Agr Biotechnol Res Ctr, Taipei, Taiwan
[8] China Med Univ, Sch Chinese Med, Taichung, Taiwan
[9] Ind Technol Res Inst, Biomed Technol & Device Res Labs, Hsinchu, Taiwan
关键词
GROWTH-FACTOR; DOWN-REGULATION; METASTASIS; NRF2; CARCINOMA; PROGRESSION; SUPPRESSOR; OLTIPRAZ; INVASION; RECEPTOR;
D O I
10.1038/s41598-019-49874-8
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cell migration is a fundamental feature of cancer recurrence. Since recurrence is correlated with high mortality in lung cancer, it follows that reducing cell migration would decrease recurrence and increase survival rates. Semaphorin-6A (SEMA6A), a protein initially known as a regulator of axonal guidance, is down-regulated in lung cancer tissue, and low levels of SEMA6A are associated with cancer recurrence. Thus, we hypothesized that SEMA6A could suppress cancer cell migration. In this study, we found that the migration capability of H1299 lung cancer cells decreased with SEMA6A overexpression, while it increased with SEMA6A silencing. Moreover, silencing of the cellular homeostasis protein Heme-oxygenase-1 (HMOX1) and/or the transcription factor Nuclear Factor, Erythroid-2-Like-2 (NRF2) reversed the migration-suppressing effect of SEMA6A and the SEMA6A-driven alterations in expression of urokinase insulin-like-growth-factor-binding-protein-3, Matrix metalloproteinase (MMP)-1, and MMP9, the downstream effectors of HMOX1. Taken together, these results demonstrate that SEMA6A is a potential suppressor of cancer migration that functions through the NRF2/HMOX1 axis. Our results explain why low SEMA6A is linked to high recurrence in the clinical setting and suggest that SEMA6A could be useful as a biomarker or target in lung cancer therapy.
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页数:9
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