Nondestructive analysis of single rapeseeds by means of Raman spectroscopy

The value of different vegetable oils can be correlated with the content of polyunsaturated fatty acids, especially omega-3-fatty acids such as linolenic acid, because of their contribution to healthy nutrition. One expression for the degree of unsaturation is the iodine value normally measured with gas chromatography. The use of Raman spectroscopy allows a rapid calculation of the iodine value and, in addition, only in a minimal sample volume. Therefore, this method can be used in single rapeseeds in order to predict the iodine value before harvesting. Additionally, the method can also be used for breeding investigations. Here, the lipid content and composition of a plant can be predicted by measuring the seedling without destruction. Copyright (C) 2006 John Wiley & Sons, Ltd.