New orthotopic implantation model of human esophageal squamous cell carcinoma in athymic nude mice
被引:15
作者:
Song, Shuai
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Capital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R ChinaCapital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
Song, Shuai
[1
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Chang, Dong
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Capital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R ChinaCapital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
Chang, Dong
[1
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Cui, Yong
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Capital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R ChinaCapital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
Cui, Yong
[1
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Hu, Jian
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Capital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R ChinaCapital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
Hu, Jian
[1
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Gong, Min
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Capital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R ChinaCapital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
Gong, Min
[1
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Ma, Kai
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Qingdao Univ, Affiliated Hosp, Dept Thorac Surg, Qingdao 266071, Peoples R ChinaCapital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
Ma, Kai
[2
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Ding, Fang
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机构:
Chinese Acad Med Sci, Canc Inst & Hosp, State Key Lab Mol Oncol, Beijing 100730, Peoples R China
Peking Union Med Coll, Beijing 100021, Peoples R ChinaCapital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
Ding, Fang
[3
,4
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Liu, Zhi-Hua
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机构:
Chinese Acad Med Sci, Canc Inst & Hosp, State Key Lab Mol Oncol, Beijing 100730, Peoples R China
Peking Union Med Coll, Beijing 100021, Peoples R ChinaCapital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
Liu, Zhi-Hua
[3
,4
]
Wang, Tian-You
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Capital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R ChinaCapital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
Wang, Tian-You
[1
]
机构:
[1] Capital Med Univ, Beijing Friendship Hosp, Dept Thorac Surg, Beijing 100050, Peoples R China
[2] Qingdao Univ, Affiliated Hosp, Dept Thorac Surg, Qingdao 266071, Peoples R China
[3] Chinese Acad Med Sci, Canc Inst & Hosp, State Key Lab Mol Oncol, Beijing 100730, Peoples R China
[4] Peking Union Med Coll, Beijing 100021, Peoples R China
Background: Subcutaneous xenograft is a common method to establish animal models of human esophageal squamous cell carcinoma (ESCC). However, the growth microenvironment of transplanted tumors is different from primary tumors. Orthotopic implantation models can provide more biologically relevant context in which to study the disease. So far, an orthotopic implantation model of ESCC has rarely been reported. Methods: The human ESCC cell line KYSE30 was transfected with pLVX-Luciferase plasmids. KYSE30-Luciferase cells were isolated and injected into the flanks of nude mice to develop a subcutaneous tumor. An orthotopic implantation model was established using the fragments derived from the subcutaneous tumor. Fluorescence imaging was used to observe the development of the orthotopic implanted tumor. Hematoxylin and eosin staining was performed to evaluate the invasion and metastasis of the tumor. Results: KYSE30 cells were successfully transfected with pLVX-Luciferase plasmids. A primary tumor was developed in all mice. The mice experienced body weight loss. The implanted tumor infiltrated into the esophageal muscularis propria. However, neither distant organ nor lymph node metastasis was found. The progression of the primary tumor was monitored by in vivo fluorescence imaging. Conclusion: The orthotopic implantation model can be established by sewing the fragments of human ESCC to the abdominal esophagus of a nude mouse. The progression of an orthotopic implantation tumor can be monitored in real time by in vivo fluorescence imaging.
机构:
Univ Bradford, Tom Connors Canc Res Ctr, Bradford BD7 1DP, W Yorkshire, EnglandUniv Bradford, Tom Connors Canc Res Ctr, Bradford BD7 1DP, W Yorkshire, England
机构:
Univ Bradford, Tom Connors Canc Res Ctr, Bradford BD7 1DP, W Yorkshire, EnglandUniv Bradford, Tom Connors Canc Res Ctr, Bradford BD7 1DP, W Yorkshire, England