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Exploration of genetically encoded voltage indicators based on a chimeric voltage sensing domain
被引:0
作者:
Mishina, Yukiko
[1
,2
]
Mutoh, Hiroki
[1
,3
]
Song, Chenchen
[4
]
Knoepfel, Thomas
[1
,4
]
机构:
[1] RIKEN, Brain Sci Inst, Lab Neuronal Circuit Dynam, Wako, Saitama, Japan
[2] Univ Tokyo, Ctr Global Commun Strategies, Tokyo, Japan
[3] Hamamatsu Univ, Sch Med, Dept Neurophysiol, Shizuoka, Japan
[4] Univ London Imperial Coll Sci Technol & Med, Dept Med, Div Brain Sci, London W12 0NN, England
关键词:
optogenetics;
mouse model;
optical imaging;
voltage imaging;
FRET sensors;
ELECTRICAL-ACTIVITY;
OPTICAL INDICATORS;
SENSORY RESPONSES;
ACTION-POTENTIALS;
LAYER;
2/3;
IN-VIVO;
DYNAMICS;
FRET;
NEURONS;
BRIGHT;
D O I:
10.3389/fnmo1.2014.00078
中图分类号:
Q189 [神经科学];
学科分类号:
071006 ;
摘要:
Deciphering how the brain generates cognitive function from patterns of electrical signals is one of the ultimate challenges in neuroscience. To this end, it would be highly desirable to monitor the activities of very large numbers of neurons while an animal engages in complex behaviors. Optical imaging of electrical activity using genetically encoded voltage indicators (GEVIs) has the potential to meet this challenge. Currently prevalent GEVIs are based on the voltage-sensitive fluorescent protein (VSFP) prototypical design or on the voltage-dependent state transitions of microbial opsins. We recently introduced a newVSFP design in which the voltage-sensing domain (VSD) is sandwiched between a fluorescence resonance energy transfer pair of fluorescent proteins (termed VSFP-Butterflies) and also demonstrated a series of chimeric VSD in which portions of the VSD of Ciona intestinalis voltage-sensitive phosphatase are substituted by homologous portions of a voltage-gated potassium channel subunit. These chimericVSD had faster sensing kinetics than that of the native Ci-VSD. Here, we describe a new set of VSFPs that combine chimeric VSD with the Butterfly structure. We show that these chimeric VSFP-Butterflies can report membrane voltage oscillations of up to 200 Hz in cultured cells and report sensory evoked cortical population responses in living mice. This class of GEVIs may be suitable for imaging of brain rhythms in behaving mammalians.
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页数:8
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