The effects of thymoquinone on hippocampal cytokine level, brain oxidative stress status and memory deficits induced by lipopolysaccharide in rats

被引:84
作者
Bargi, Rahimeh [1 ]
Asgharzadeh, Fereshteh [2 ]
Beheshti, Farimah [3 ]
Hosseini, Mahmoud [1 ]
Sadeghnia, Hamid Reza [4 ]
Khazaei, Majid [2 ]
机构
[1] Mashhad Univ Med Sci, Psychiat & Behav Sci Res Ctr, Div Neurocognit Sci, Mashhad, Iran
[2] Mashhad Univ Med Sci, Neurogen Inflammat Res Ctr, Mashhad, Iran
[3] Mashhad Univ Med Sci, Dept Physiol, Fac Med, Mashhad, Iran
[4] Mashhad Univ Med Sci, Pharmacol Res Ctr Med Plants, Mashhad, Iran
关键词
Rat; Lipopolysaccharide; Thymoquinone; Memory; Oxidative damage; Cytokine; HYDRO-ALCOHOLIC EXTRACT; LONG-TERM POTENTIATION; NIGELLA-SATIVA; SPATIAL MEMORY; ALZHEIMERS-DISEASE; NITRIC-OXIDE; TNF-ALPHA; IMPAIRMENT; ANTIOXIDANT; EXPOSURE;
D O I
10.1016/j.cyto.2017.04.015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Objective: The study objective was to determine the protective effects of thymoquinone (TQ) on brain tissues oxidative stress status, hippocampal cytokine level, and learning and memory deficits induced by lipopolysaccharide (LPS) in rats. Methods: Animals were randomly divided into the following groups and treated: (1) Control (saline), (2) LPS (1 mg/kg i.p.), (3-5) 2, 5 or 10 mg/kg TQ extract 30 min before LPS injection. The treatment was started since two weeks before the behavioral experiments and continued during the behavioral tests (LPS injected 2 h before each behavioral experiment). Finally, the brains were removed for biochemical assessments. Results: Morris water maze (MWM) test results showed that LPS increased escape latency compared to control group whereas TQ decreased them vs. LPS group. In passive avoidance (PA) test, LPS reduced the latency to enter the dark compartment vs. control group, while TQ treatment attenuated this effect of LPS. Additionally, LPS increased interleukin-6 (IL-6) and tumor necrosis alpha (TNF-alpha) in the hippocampal tissues. It also elevated malondialdehyde (MDA) and nitric oxide (NO) metabolites and decreased thiol content, superoxide dismutase (SOD) and catalase (CAT) in both hippocampus and cortex vs. control group, while TQ decreased IL-6, TNF-alpha, MDA and NO metabolites and increased thiol content, SOD and CAT compared to LPS group. C Conclusion: Findings of current study indicated that TQ improved LPS-induced learning and memory impairments induced by LPS in rats by attenuating the hippocampal cytokine levels and brain tissues oxidative damage.
引用
收藏
页码:173 / 184
页数:12
相关论文
共 68 条
[1]   A potential mechanism for the ameliorative effect of thymoquinone on pentylenetetrazole-induced kindling and cognitive impairments in mice [J].
Abdel-Zaher, Ahmed O. ;
Farghaly, Hanan S. M. ;
Farrag, Magda M. Y. ;
Abdel-Rahman, Mahran S. ;
Abdel-Wahab, Basel A. .
BIOMEDICINE & PHARMACOTHERAPY, 2017, 88 :553-561
[2]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[3]   Inducible nitric oxide inhibitor aminoguanidine, ameliorates deleterious effects of lipopolysaccharide on memory and long term potentiation in rat [J].
Anaeigoudari, Akbar ;
Soukhtanloo, Mohammad ;
Reisi, Parham ;
Beheshti, Farimah ;
Hosseini, Mahmoud .
LIFE SCIENCES, 2016, 158 :22-30
[4]   Hippocampal interleukin-1β gene expression during long-term potentiation decays with age [J].
Balschun, D ;
Randolf, A ;
Pitossi, F ;
Schneider, H ;
Del Rey, A ;
Besedovsky, HO .
NEUROENDOCRINE AND NEURAL REGULATION OF AUTOIMMUNE AND INFLAMMATORY DISEASE: MOLECULAR, SYSTEMS, AND CLINICAL INSIGHTS, 2003, 992 :1-8
[5]   Peripheral infection and aging interact to impair hippocampal memory consolidation [J].
Barrientos, RM ;
Higgins, EA ;
Biedenkapp, JC ;
Sprunger, DB ;
Wright-Hardesty, KJ ;
Watkins, LR ;
Rudy, JW ;
Maier, SF .
NEUROBIOLOGY OF AGING, 2006, 27 (05) :723-732
[6]   Memory for context is impaired by a post context exposure injection of interleukin-1 beta into dorsal hippocampus [J].
Barrientos, RM ;
Higgins, EA ;
Sprunger, DB ;
Watkins, LR ;
Rudy, JW ;
Maier, SF .
BEHAVIOURAL BRAIN RESEARCH, 2002, 134 (1-2) :291-298
[7]   Reactive Oxygen Species: Physiological and Physiopathological Effects on Synaptic Plasticity [J].
Beckhauser, Thiago Fernando ;
Francis-Oliveira, Jose ;
De Pasquale, Roberto .
JOURNAL OF EXPERIMENTAL NEUROSCIENCE, 2016, 10 :23-48
[8]   The effects of Nigella sativa extract on hypothyroidism-associated learning and memory impairment during neonatal and juvenile growth in rats [J].
Beheshti, Farimah ;
Hosseini, Mahmoud ;
Shafei, Mohammad Naser ;
Soukhtanloo, Mohammad ;
Ghasemi, Simagol ;
Vafaee, Farzaneh ;
Zarepoor, Leila .
NUTRITIONAL NEUROSCIENCE, 2017, 20 (01) :49-59
[9]  
Beheshti Farimah, 2016, J Tradit Complement Med, V6, P146, DOI 10.1016/j.jtcme.2014.11.039
[10]  
Beheshti F, 2016, AVICENNA J PHYTOMEDI, V6, P124