Home-brew FISH assay shows higher efficiency than BCR-ABL dual color, dual fusion probe in detecting microdeletions and complex rearrangements associated with t(9;22) in chronic myeloid leukemia

被引：23
作者：
Albano, Francesco
Anelli, Luisa
Zagaria, Antonella
Archidiacono, Nicoletta
Liso, Vincenzo
Specchia, Giorgina
Rocchi, Mariano
机构：
[1] Univ Bari, Dept Hematol, Policlin, I-70124 Bari, Italy
[2] Univ Bari, Dipartimento Genet & Microbiol, Policlin, I-70124 Bari, Italy
来源：
CANCER GENETICS AND CYTOGENETICS | 2007年 / 174卷 / 02期
关键词：
D O I：
10.1016/j.cancergencyto.2006.09.025
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We carried out fluorescence in situ hybridization (FISH) studies on 18 Ph+ chronic myeloid leukemia (CML) cases with chromosome 22 genomic deletions with the Vysis BCR-ABL dual-color/dual-fusion probe (BCR-ABL DC/DF) to compare the hybridization patterns obtained with this approach to those obtained with the "home brew" BAC/PAC system. Our results are the following: chromosome 22 microdeletions less than 400 kilobases (Kb) were not detected by the BCR DC/DF probe; FISH analysis with the BCR DC/DF probe in cases bearing chromosome 22 microdeletions ranging from 400 to 700 Kb produced a faint signal on the der(9); and the BCR-ABL DC/DF FISH pattern was comparable to the one obtained by the home brew probe in the presence of a 900-Kb chromosome 22 microdeletion. Our home-brew FISH system represents an accurate method for revealing a subset of CML patients with der(9) microdeletions. (c) 2007 Elsevier Inc. All rights reserved.
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页码：121 / 126
页数：6
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