What vibrations tell us about proteins

被引：1717

作者：

Barth, A ^{[1
]}

Zscherp, C ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Inst Biophys, D-60590 Frankfurt, Germany

来源：

QUARTERLY REVIEWS OF BIOPHYSICS | 2002年 / 35卷 / 04期

关键词：

D O I：

10.1017/S0033583502003815

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This review deals with current concepts of vibrational spectroscopy for the investigation of protein structure and function. While the focus is on infrared (IR) spectroscopy, some of the general aspects also apply to Raman spectroscopy. Special emphasis is on the amide 1 vibration of the polypeptide backbone that is used for secondary-structure analysis. Theoretical as well as experimental aspects are covered including transition dipole coupling. Further topics are discussed, namely the absorption of amino-acid side-chains, H-1/H-2 exchange to study the conformational flexibility and reaction-induced difference spectroscopy for the investigation of reaction mechanisms with a focus on interpretation tools.

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页码：369 / 430

页数：62

相关论文

共 275 条

[111] Conformational mapping of the N-terminal segment of surfactant protein B in lipid using 13C-enhanced Fourier transform infrared spectroscopy [J].

Gordon, LM ;

Lee, KYC ;

Lipp, MM ;

Zasadzinski, JA ;

Walther, FJ ;

Sherman, MA ;

Waring, AJ .

JOURNAL OF PEPTIDE RESEARCH, 2000, 55 (04) :330-347

[112] Characterization of the primary electron donor of photosystem I, P700, by electrochemistry and Fourier transform infrared (FTIR) difference spectroscopy [J].

Hamacher, E ;

Kruip, J ;

Rogner, M ;

Mantele, W .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 1996, 52 (01) :107-121

[113] Structure of the amide I band of peptides measured by femtosecond nonlinear-infrared spectroscopy [J].

Hamm, P ;

Lim, MH ;

Hochstrasser, RM .

JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (31) :6123-6138

[114] The two-dimensional IR nonlinear spectroscopy of a cyclic penta-peptide in relation to its three-dimensional structure [J].

Hamm, P ;

Lim, M ;

DeGrado, WF ;

Hochstrasser, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (05) :2036-2041

[115]

Haris P I, 1994, Methods Mol Biol, V22, P183

[116] POTENTIAL OF C-13 AND N-15 LABELING FOR STUDYING PROTEIN-PROTEIN INTERACTIONS USING FOURIER-TRANSFORM INFRARED-SPECTROSCOPY [J].

HARIS, PI ;

ROBILLARD, GT ;

VANDIJK, AA ;

CHAPMAN, D .

BIOCHEMISTRY, 1992, 31 (27) :6279-6284

[117] Vibrational spectra and ab initio DFT calculations of 4-methylimidazole and its different protonation forms: Infrared and raman markers of the protonation state of a histidine side chain [J].

Hasegawa, K ;

Ono, T ;

Noguchi, T .

JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (17) :4253-4265

[118] Interpretation of amide I difference bands observed during protein reactions using site-directed isotopically labeled bacteriorhodopsin as a model system [J].

Hauser, K ;

Engelhard, M ;

Friedman, N ;

Sheves, M ;

Siebert, F .

JOURNAL OF PHYSICAL CHEMISTRY A, 2002, 106 (14) :3553-3559

[119] FTIR-spectroscopy of multistranded coiled coil proteins [J].

Heimburg, T ;

Schünemann, J ;

Weber, K ;

Geisler, N .

BIOCHEMISTRY, 1999, 38 (39) :12727-12734

[120] Vibrational modes of ubiquinone in cytochrome bo3 from Escherichia coli identified by Fourier transform infrared difference spectroscopy and specific 13C labeling [J].

Hellwig, P ;

Mogi, T ;

Tomson, FL ;

Gennis, RB ;

Iwata, J ;

Miyoshi, H ;

Mäntele, W .

BIOCHEMISTRY, 1999, 38 (44) :14683-14689

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