A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

被引:42
作者
Bakthavatsalam, Subha [1 ]
Dodo, Kosuke [1 ,2 ]
Sodeoka, Mikiko [1 ,2 ]
机构
[1] RIKEN Cluster Pioneering Res, Synthet Organ Chem Lab, Wako, Saitama 3510198, Japan
[2] RIKEN Ctr Sustainable Resource Sci, 2-1 Hirosawa, Wako, Saitama 3510198, Japan
来源
RSC CHEMICAL BIOLOGY | 2021年 / 2卷 / 05期
关键词
SINGLE LIVING CELLS; SCATTERING MICROSCOPY; IN-VIVO; LIVE CELLS; VIRULENCE FACTOR; CLICK CHEMISTRY; PROBE; EXCITATION; TISSUES; IDENTIFICATION;
D O I
10.1039/d1cb00116g
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alkyne functional groups have Raman signatures in a region (1800 cm(-1) to 2800 cm(-1)) that is free from interference from cell components, known as the "silent region", and alkyne signals in this region were first utilized a decade ago to visualize the nuclear localization of a thymidine analogue EdU. Since then, the strategy of Raman imaging of biological samples by using alkyne functional groups, called alkyne-tag Raman imaging (ATRI), has become widely used. This article reviews the applications of ATRI in biological samples ranging from organelles to whole animal models, and briefly discusses the prospects for this technique.
引用
收藏
页码:1415 / 1429
页数:15
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