The Inhibitory Effect of Baicalin on the Short-Term Food Intake in C57BL/6J Mice

被引：15

作者：

Kim, Eun-Ho ^{[1
]}

Son, Rak-Ho ^{[1
]}

Myoung, Hyeon-Jong ^{[1
]}

Mar, Woongchon ^{[2
]}

Kim, Won-Ki ^{[3
]}

Nam, Kung-Woo ^{[1
,2
,3
]}

机构：

[1] Jung San Bio Technol, Hwaseong 445964, South Korea

[2] Seoul Natl Univ, Inst Nat Prod Res, Coll Pharm, Seoul 151742, South Korea

[3] Korea Univ, Dept Neurosci, Coll Med, Seoul 136701, South Korea

来源：

BIOMOLECULES & THERAPEUTICS | 2010年 / 18卷 / 02期

关键词：

Baicalin; Scutellaria baicalensis; POMC; CART; Food intake; NERVOUS-SYSTEM CONTROL; RECEPTOR AGONISTS; NEUROPEPTIDE-Y; AGOUTI; HYPOTHALAMUS; SCUTELLARIA; EXPRESSION; PEPTIDE;

D O I：

10.4062/biomolther.2010.18.2.171

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Appetite is inhibited by the anorexigenic neuropeptides POMC (proopiomelanocortin) and CART (cocaine-amphetamine-regulated transcript) in the hypothalamus. The present study was performed to examine the inhibitory effects of baicalin against food intake and the upregulation of POMC/CART. Short-term food intake (48 h) was significantly inhibited by treatment with baicalin (10 mg/kg, p < 0.05) in C57BL/6 mice. Immunohistochemical analysis showed that baicalin upregulated POMC and CART levels in the arcuate nucleus of the hypothalamus. These effects were also examined using an in vitro system. pPOMC-Luc or pCART-Luc plasmids were transformed into mouse N29-2 neuronal and human SH-SY5Y cells, and the activities of baicalin were examined in these cells. Baicalin increased POMC and CART promoter-driven luciferase activity in a dose-dependent manner without cytotoxic effects. These results suggest that baicalin downregulates short-term food intake while upregulating POMC and CART expression.

引用

页码：171 / 177

页数：7

共 30 条

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