Isolation and Characterization of Twenty-four Microsatellite Loci for Rhododendron decorum Franch. (Ericaceae)

被引:14
|
作者
Wang, Xue-qin [1 ,2 ]
Huang, Yuan [1 ]
Long, Chun-lin [1 ,3 ]
机构
[1] Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
[3] Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
基金
日本学术振兴会;
关键词
Rhododendron decorum; microsatellite; genetic diversity; NATURAL HYBRIDIZATION; MOLECULAR EVIDENCE;
D O I
10.21273/HORTSCI.44.7.2028
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Rhododendron decorum is a common species in southwest China and northeast Myanmar, in which the flowers have been eaten as a favorite vegetable. We isolated and characterized 24 microsatellite primer pairs from this species. The number of alleles ranged from two to seven. The observed and expected heterozygosities (H-O and H-E) were 0.3830 to 0.7855 and 0 to 0.7917, respectively. Eleven loci were significantly deviated from Hardy-Weinberg equilibrium as a result of the heterozygote deficiency. Cross-species amplification in another eight Rhododendron species showed their potential use for evolutionary and conservation studied in this genus. These markers will be useful to reveal the genetic population structure and genetic diversity of R. decorum.
引用
收藏
页码:2028 / 2030
页数:3
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