Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize

被引：97
作者：
Masarik, M
Kizek, R
Kramer, KJ
Billova, S
Brazdova, M
Vacek, J
Bailey, M
Jelen, F
Howard, JA
机构：
[1] Acad Sci Czech Republ, Inst Biophys, CZ-61265 Brno, Czech Republic
[2] Mendel Univ Agr & Forestry, Dept Chem & Biochem, Brno 61300, Czech Republic
[3] USDA ARS, Grain Mkt & Prod Res Ctr, Manhattan, KS 66502 USA
[4] ProdiGene, College Stn, TX 77843 USA
来源：
ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 11期
关键词：
D O I：
10.1021/ac020788z
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quatifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
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页码：2663 / 2669
页数：7
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