Stretchable ECM Patch Enhances Stem Cell Delivery for Post-MI Cardiovascular Repair

被引:33
作者
Kim, In Gul [1 ,2 ]
Hwang, Mintai P. [1 ,3 ]
Park, Jin Sil [4 ]
Kim, Su-Hyun [5 ]
Kim, Jung-Hyun [4 ]
Kang, Hyo Jin [4 ]
Subbiah, Ramesh [1 ]
Ko, Ung Hyun [6 ]
Shin, Jennifer H. [6 ]
Kim, Chong-Hyun [5 ]
Choi, Donghoon [4 ]
Park, Kwideok [1 ,7 ]
机构
[1] KIST, Ctr Biomat, Seoul 02792, South Korea
[2] Seoul Natl Univ Hosp, Dept Otorhinolaryngol Head & Neck Surg, Seoul 03080, South Korea
[3] Cornell Univ, Meinig Sch Biomed Engn, Ithaca, NY 14853 USA
[4] Yonsei Univ Hlth Syst, Severance Cardiovasc Hosp, Seoul 03722, South Korea
[5] KIST, Ctr Neurosci, Seoul 02792, South Korea
[6] Korea Adv Inst Sci & Technol, Dept Mech Engn, Daejeon 34141, South Korea
[7] UST, Div Biomed Sci & Technol, Seoul 02792, South Korea
基金
新加坡国家研究基金会;
关键词
cardiac patches; extracellular matrix (ECM); mesenchymal stem cells; myocardial infarction; polyvinyl alcohol (PVA); EXTRACELLULAR-MATRIX; ISCHEMIC-HEART; STROMAL CELLS; IN-VITRO; SCAFFOLD; THERAPY; OSTEOGENESIS; MECHANISMS; INFARCTION; APOPTOSIS;
D O I
10.1002/adhm.201900593
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Current cell-based therapies administered after myocardial infarction (MI) show limited efficacy due to subpar cell retention in a dynamically beating heart. In particular, cardiac patches generally provide a cursory level of cell attachment due to the lack of an adequate microenvironment. From this perspective, decellularized cell-derived ECM (CDM) is attractive in its recapitulation of a natural biophysical environment for cells. Unfortunately, its weak physical property renders it difficult to retain in its original form, limiting its full potential. Here, a novel strategy to peel CDM off from its underlying substrate is proposed. By physically stamping it onto a polyvinyl alcohol hydrogel, the resulting stretchable extracellular matrix (ECM) membrane preserves the natural microenvironment of CDM, thereby conferring a biological interface to a viscoelastic membrane. Its various mechanical and biological properties are characterized and its capacity to improve cardiomyocyte functionality is demonstrated. Finally, evidence of enhanced stem cell delivery using the stretchable ECM membrane is presented, which leads to improved cardiac remodeling in a rat MI model. A new class of material based on natural CDM is envisioned for the enhanced delivery of cells and growth factors that have a known affinity with ECM.
引用
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页数:13
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