Comparative analysis of LytS/LytTR-type histidine kinase/response regulator systems in γ-proteobacteria

被引：15
作者：
Behr, Stefan [1 ]
Brameyer, Sophie [1 ]
Witting, Michael [2 ,3 ]
Schmitt-Kopplin, Philipp [2 ,3 ]
Jung, Kirsten [1 ]
机构：
[1] Ludwig Maximilians Univ Munchen, Dept Microbiol, Munich Ctr Integrated Prot Sci CIPSM, Martinsried, Germany
[2] Helmholtz Zentrum Munchen, Deutsch Forschungszentrum Gesundheit & Umwelt Gmb, Res Unit Analyt BioGeoChem, Neuherberg, Germany
[3] Tech Univ Munich, Wissensch Zentrum Weihenstephan Ernahrung Landnut, Lehrstuhl Analyt Lebensmittelchem, Freising Weihenstephan, Germany
来源：
PLOS ONE | 2017年 / 12卷 / 08期
关键词：
IDENTIFICATION; BACTERIA; DATABASE;
D O I：
10.1371/journal.pone.0182993
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Bacterial histidine kinase/response regulator systems operate at the interface between environmental cues and physiological states. Escherichia coli contains two LytS/LytTR-type histidine kinase/response regulator systems, BtsS/BtsR (formerly YehU/YehT) and YpdA/YpdB, which have been identified as pyruvate-responsive two-component systems. Since they exhibit remarkable similarity, we analyzed their phylogenetic distribution within the gamma-proteobacteria, and experimentally characterized them in a set of representative species. We found that BtsS/BtsR is the predominant LytS/LytTR-type two-component system among gamma-proteobacteria, whereas YpdA/YpdB primarily appears in a supplementary role. Based on our observations in E. coli, we used the highly conserved DNA-binding motifs to test the in vivo functionality of both systems in various genera, including Salmonella, Enterobacter, Citrobacter, Xenorhabdus, Yersinia, Aeromonas and Vibrio. The results suggest that, in all cases tested, BtsS/BtsR and YpdA/YpdB respond to different levels of pyruvate in the environment.
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页数：14
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